Evidence map›Paper›PMID 25792974›Full record

ArticleCancer cell international2015

Downregulated lincRNA HOTAIR expression in ovarian cancer stem cells decreases its tumorgeniesis and metastasis by inhibiting epithelial-mesenchymal transition.

Jing Wang, Dengyu Chen, Xiangfeng He, Yuxia Zhang, Fangfang Shi, Di Wu, Junsong Chen, Ying Zhang, Fengsu Zhao, Jun Dou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 2 pooled it
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 2 syntheses or guidelines pooled it, 41 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Long non-coding RNA H19 promotes tumorigenesis of ovarian cancer by sponging miR-675.International journal of clinical and experimental pathology · 2019
    Article
  13. Article
  14. Article
  15. Autophagy maintains the stemness of ovarian cancer stem cells by FOXA2.Journal of experimental & clinical cancer research : CR · 2017
    Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. The Role of Long Non-Coding RNAs in Ovarian Cancer.Iranian biomedical journal · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Jing Wang *Department of Gynecology & Obstetrics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009 China.
Dengyu Chen *Department of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.
Xiangfeng He *Department of Medical Oncology, Affiliated Tumor Hospital of Nantong University, Nantong, 226361 China.
Yuxia ZhangDepartment of Gynecology & Obstetrics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009 China.
Fangfang ShiDepartment of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.
Di WuDepartment of Gynecology & Obstetrics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009 China.
Junsong ChenDepartment of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.
Ying ZhangDepartment of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.
Fengsu ZhaoDepartment of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.
Jun DouDepartment of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.
Nantong University · CNZhongda Hospital Southeast University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEmerging evidence indicates that dysregulated long intervening non-coding RNA (lincRNA) HOTAIR correlates highly with tumor invasion and metastasis but a link between the high expression of HOTAIR and the metastatic cascade of cancer stem cells (CSCs) needs to be further studied. The purpose of this study was to investigate the effect of down-regulated HOTAIR expression on tumorgeniesis and metastasis of epithelial ovarian cancer (EOC) CSCs. CD117(+)CD44(+)CSCs were isolated from human EOC SKOV3 cell line by using a magnetic-activated cell sorting system, and were then transfected with the expression vector-based small hairpin RNA targeting HOTAIR; the stably transfected cells were selected for the study. Colony-forming, wound-healing, cellular metastasis and tumorigenicity assays were performed.

resultsThe results demonstrated that the HOTAIR expression in clinical EOC tissues and SKOV3 CD117(+)CD44(+)CSCs was higher than in SKOV3 tumor tissues and non-CD117(+)CD44(+)CSCs. The CD117(+)CD44(+)-shHOTAIR showed an inhibited HOTAIR expression, reduced cell migration and invasion than CD117(+)CD44(+)- scramble, suggesting the inhibition of an epithelial-mesenchymal transition. Moreover, the downregulated HOTAIR expression in CD117(+)CD44(+) CSCs significantly decreased the tumor growth and lung metastasis in xenograft mice.

conclusionOur findings demonstrated the shHOTAIR-mediated down-regulation of the HOTAIR expression in CD117(+)CD44(+) CSCs can be a promising new opportunity for future clinical trials.

Indexed as

Cancer stem cellsEpithelial-mesenchymal transitionEpithelial ovarian cancerLincRNA HOTAIRRNA interference

Identifiers

PMID25792974
PMCPMC4365532
OpenAlexW2097562171

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.