Evidence map›Paper›PMID 25792521›Full record

ArticleJournal of dental research2015

Exon4 amelogenin transcripts in enamel biomineralization.

J Stahl, Y Nakano, J Horst, L Zhu, M Le, Y Zhang, H Liu, W Li, P K Den Besten

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of dental research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

J StahlDepartment of Orofacial Sciences, University of California, San Francisco, School of Dentistry, San Francisco, CA, USA Maxillofacial Injury and Disease Department, Naval Medical Research Unit, San Antonio, TX, USA.
Y NakanoDepartment of Orofacial Sciences, University of California, San Francisco, School of Dentistry, San Francisco, CA, USA.
J HorstDepartment of Biochemistry and Biophysics, University of California, San Francisco, School of Medicine, San Francisco, CA, USA.
L ZhuDepartment of Orofacial Sciences, University of California, San Francisco, School of Dentistry, San Francisco, CA, USA.
M LeDepartment of Orofacial Sciences, University of California, San Francisco, School of Dentistry, San Francisco, CA, USA.
Y ZhangDepartment of Orofacial Sciences, University of California, San Francisco, School of Dentistry, San Francisco, CA, USA.
H LiuDepartment of Obstetrics, Gynecology & Reproductive Sciences & Sandler-Moore Mass Spectrometry Core Facility, University of California, San Francisco, CA, USA.
W LiDepartment of Orofacial Sciences, University of California, San Francisco, School of Dentistry, San Francisco, CA, USA.
P K Den BestenDepartment of Orofacial Sciences, University of California, San Francisco, School of Dentistry, San Francisco, CA, USA pamela.denbesten@ucsf.edu.
University of California, San Francisco · US

Funding

Amelogenin Degradation by MMP20 in Enamel mineralizationR01DE015821 · NIDCR · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LI, WU · 2004 to 2014
$3.3M
Molecular/Mechanical Imaging of Dentin Bonding SubstrateK25DE015281 · NIDCR · UNIVERSITY OF MISSOURI KANSAS CITY · PI WANG, YONG · 2003 to 2007
$566k
NIDCR NIH HHS 2R01DE015821NIDCR NIH HHS 3R01DE015281S1NIDCR NIH HHS K25 DE015281NIDCR NIH HHS R01 DE015821
6 · The paper itself

Abstract

Amelogenins are proteins formed by alternative splicing of the amelogenin gene, and are essential for tooth enamel formation. However, the unique functions of various alternatively spliced amelogenins in enamel formation are not well understood. In this study, we determined the spatiotemporal location of amelogenins derived from transcripts containing exon4 (AMG+4) in the enamel matrix, and the relative binding of recombinant AMG+4 to hydroxyapatite (HAP). Immunohistochemistry and mass spectrometry analyses showed that AMG+4 proteins were secreted into the enamel matrix at the early maturation stage. A stage-specific increase in the synthesis of AMG+4 was further supported by our observation that in mice overexpressing leucine-rich amelogenin peptide (TgLRAP), in which ameloblasts differentiate earlier, AMG+4 transcripts were also upregulated earlier. In vitro binding studies, supported by in silico modeling of protein binding to calcium and phosphate, showed that more recombinant AMG+4 bound to hydroxyapatite (HAP) as compared with recombinant AMG-4. The temporal and spatial localization of amelogenins containing exon4 peptide, and their functional differences in HAP binding, suggests that the unique properties of amelogenins containing exon4 cause a specific enhancement of biomineralization related to stabilization of early-formed HAP at the maturation stage.

Indexed as

Alternative SplicingAmeloblastsAmelogenesisAmelogeninAnimalsCalciumCell DifferentiationDental Enamel ProteinsDurapatiteExonsFemaleMass SpectrometryMicePhosphatesProtein BindingRatsAmelogeninCalciumDental Enamel ProteinsDurapatiteleucine-rich amelogenin peptidePhosphatesRecombinant Proteinscell-matrix interactionscraniofacial biologymatrix biologymineralized tissueprotein expressiontooth development

Identifiers

PMID25792521
PMCPMC4485327
OpenAlexW1982538611

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.