Evidence map›Paper›PMID 25792253›Full record

ArticlePlant physiology2015

The Arabidopsis synaptotagmin1 is enriched in endoplasmic reticulum-plasma membrane contact sites and confers cellular resistance to mechanical stresses.

Jessica Pérez-Sancho, Steffen Vanneste, Eunkyoung Lee, Heather E McFarlane, Alicia Esteban Del Valle, Victoriano Valpuesta, Jiří Friml, Miguel A Botella, Abel Rosado

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.

0numbers the graph read from it
0cells of the map it votes in
80citing papers in PubMed
8.0field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

80 citing papers in PubMed, 167 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Concerted transport and phosphorylation of diacylglycerol at ER-PM contact sites regulate phospholipid dynamics during stress.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  7. The Extended Synaptotagmins ofPlants (Basel, Switzerland) · 2025
    Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Organelle Interactions in Plant Cells.Results and problems in cell differentiation · 2024
    Review
  17. Article
  18. Review
  19. Topological damping in an ultrafast giant cell.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  20. Article

20 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Jessica Pérez-SanchoInstituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
Steffen VannesteInstituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
Eunkyoung LeeInstituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
Heather E McFarlaneInstituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
Alicia Esteban Del ValleInstituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
Victoriano ValpuestaInstituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
Jiří FrimlInstituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
Miguel A BotellaInstituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.) abel.rosado@botany.ubc.ca mabotella@uma.es.ORCID http://orcid.org/0000-0002-8867-1831
Abel RosadoInstituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.) abel.rosado@botany.ubc.ca mabotella@uma.es.ORCID http://orcid.org/0000-0001-8674-1050
Consejo Superior de Investigaciones Científicas · ESVlaams Instituut voor Biotechnologie · BEUniversity of British Columbia · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Eukaryotic endoplasmic reticulum (ER)-plasma membrane (PM) contact sites are evolutionarily conserved microdomains that have important roles in specialized metabolic functions such as ER-PM communication, lipid homeostasis, and Ca(2+) influx. Despite recent advances in knowledge about ER-PM contact site components and functions in yeast (Saccharomyces cerevisiae) and mammals, relatively little is known about the functional significance of these structures in plants. In this report, we characterize the Arabidopsis (Arabidopsis thaliana) phospholipid binding Synaptotagmin1 (SYT1) as a plant ortholog of the mammal extended synaptotagmins and yeast tricalbins families of ER-PM anchors. We propose that SYT1 functions at ER-PM contact sites because it displays a dual ER-PM localization, it is enriched in microtubule-depleted regions at the cell cortex, and it colocalizes with Vesicle-Associated Protein27-1, a known ER-PM marker. Furthermore, biochemical and physiological analyses indicate that SYT1 might function as an electrostatic phospholipid anchor conferring mechanical stability in plant cells. Together, the subcellular localization and functional characterization of SYT1 highlights a putative role of plant ER-PM contact site components in the cellular adaptation to environmental stresses.

Indexed as

Adaptation, PhysiologicalStress, MechanicalArabidopsisArabidopsis ProteinsCell MembraneEndoplasmic ReticulumMicrotubulesModels, BiologicalPhosphatidylinositol PhosphatesProtein BindingProtein Structure, TertiaryProtein TransportSynaptotagmin IArabidopsis ProteinsAt2g20990 protein, ArabidopsisPhosphatidylinositol PhosphatesSynaptotagmin I

Identifiers

PMID25792253
PMCPMC4424031
OpenAlexW1568479834

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