Evidence map›Paper›PMID 25791723›Full record

ArticleScientific reports2015

Oligomerized, filamentous surface presentation of RANTES/CCL5 on vascular endothelial cells.

Inger Øynebråten, Nicolas Barois, Trygve Bergeland, Axel M Küchler, Oddmund Bakke, Guttorm Haraldsen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 35 citations in OpenAlex.

  1. Trial
  2. mBio · 2026
    Article
  3. Article
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  7. Article
  8. Review
  9. The Heparanase Regulatory Network in Health and Disease.International journal of molecular sciences · 2021
    Review
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  11. Article
  12. Article
  13. Review
  14. Review
  15. Article
  16. Review
  17. Review
  18. Article
  19. Structural basis for oligomerization and glycosaminoglycan binding of CCL5 and CCL3.Proceedings of the National Academy of Sciences of the United States of America · 2016
    Article
  20. MicroRNAs in Hyperglycemia Induced Endothelial Cell Dysfunction.International journal of molecular sciences · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Inger Øynebråten1] Department of Pathology, Oslo University Hospital and University of Oslo, PO Box 4950 Nydalen, N-0424 Oslo, Norway [2] Centre for Immune Regulation, University of Oslo, RikshospitaletPO Box 4950 Nydalen, N-0424 Oslo, Norway.
Nicolas BaroisThe Department of Biosciences, University of Oslo, PO Box 1041 Blindern, 0316 N-Oslo, Norway.
Trygve BergelandThe Department of Biosciences, University of Oslo, PO Box 1041 Blindern, 0316 N-Oslo, Norway.
Axel M KüchlerDepartment of Pathology, Oslo University Hospital and University of Oslo, PO Box 4950 Nydalen, N-0424 Oslo, Norway.
Oddmund Bakke1] Centre for Immune Regulation, University of Oslo, RikshospitaletPO Box 4950 Nydalen, N-0424 Oslo, Norway [2] The Department of Biosciences, University of Oslo, PO Box 1041 Blindern, 0316 N-Oslo, Norway.
Guttorm Haraldsen1] Department of Pathology, Oslo University Hospital and University of Oslo, PO Box 4950 Nydalen, N-0424 Oslo, Norway [2] K. G. Jebsen Inflammation Research Centre, University of Oslo, RikshospitaletPO Box 4950 Nydalen, N-0424 Oslo, Norway.
Oslo University Hospital · NOUniversity of Oslo · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vascular endothelial cells present luminal chemokines that arrest rolling leukocytes by activating integrins. It appears that several chemokines must form higher-order oligomers to elicit proper in vivo effects, as mutants restricted to forming dimers have lost the ability to recruit leukocytes to sites of inflammation. Here, we show for the first time that the chemokine RANTES/CCL5 binds to the surface of human endothelial cells in a regular filamentous pattern. Furthermore, the filaments bound to the surface in a heparan sulfate-dependent manner. By electron microscopy we observed labeling for RANTES on membrane projections as well as on the remaining plasma membrane. Mutant constructs of RANTES restricted either in binding to heparin, or in forming dimers or tetramers, appeared either in a granular, non-filamentous pattern or were not detectable on the cell surface. The RANTES filaments were also present after exposure to flow, suggesting that they can be present in vivo. Taken together with the lacking in vivo or in vitro effects of RANTES mutants, we suggest that the filamentous structures of RANTES may be of physiological importance in leukocyte recruitment.

Indexed as

BiopolymersChemokine CCL5Endothelium, VascularHeparan SulfateHumansHuman Umbilical Vein Endothelial CellsInterferon-gammaTumor Necrosis Factor-alphaBiopolymersCCL5 protein, humanChemokine CCL5Heparan SulfateInterferon-gammaTumor Necrosis Factor-alpha

Identifiers

PMID25791723
PMCPMC4367157
OpenAlexW2087895326

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.