ArticleScientific reports2015
Isolation of high-purity myenteric plexus from adult human and mouse gastrointestinal tract.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 51 citations in OpenAlex.
- Trial
- Western diet induces iron-dependent enteric neurodegeneration via ferroptosis.The Journal of clinical investigation · 2026Article
- An exploratory in vitro co-culture of enteric neurons and smooth muscle cells demonstrates neuronal contribution to muscle layer formation.Scientific reports · 2026Article
- A novel method for culturing enteric neurons generates neurospheres containing functional myenteric neuronal subtypes.Journal of neuroscience methods · 2024Article
- Review
- Improving morphological and functional properties of enteric neuronal networks in vitro using a novel upside-down culture approach.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Intra-arterial delivery of neurospheres into isolated perfused porcine colons: a proof of concept.Biology methods & protocols · 2024Article
- BQ788 reveals glial ETBritish journal of pharmacology · 2023Article
- A combinatorial panel for flow cytometry-based isolation of enteric nervous system cells from human intestine.EMBO reports · 2023Article
- Methods to Study the Myenteric Plexus of Rat Small Intestine.Cellular and molecular neurobiology · 2023Article
- Cytological, molecular, cytogenetic, and physiological characterization of a novel immortalized human enteric glial cell line.Frontiers in cellular neuroscience · 2023Article
- Impact of cryopreservation on viability, gene expression and function of enteric nervous system derived neurospheres.Frontiers in cell and developmental biology · 2023Article
- Neuroprotective Effects of VEGF in the Enteric Nervous System.International journal of molecular sciences · 2022Article
- The gut brain in a dish: Murine primary enteric nervous system cell cultures.Neurogastroenterology and motility · 2022Review
- Isolation and transfection of myenteric neurons from mice for patch-clamp applications.Frontiers in molecular neuroscience · 2022Article
- Parkinson mice show functional and molecular changes in the gut long before motoric disease onset.Molecular neurodegeneration · 2021Article
- Disorders of the enteric nervous system - a holistic view.Nature reviews. Gastroenterology & hepatology · 2021Review
- Intestinal crypt-derived enteroid coculture in presence of peristaltic longitudinal muscle myenteric plexus.Biology methods & protocols · 2021Article
- Intra-gastrointestinal amyloid-β1-42 oligomers perturb enteric function and induce Alzheimer's disease pathology.The Journal of physiology · 2020Article
- Innervation: the missing link for biofabricated tissues and organs.NPJ Regenerative medicine · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The enteric nervous system (ENS) orchestrates a broad range of important gastrointestinal functions such as intestinal motility and gastric secretion. The ENS can be affected by environmental factors, diet and disease. Changes due to these alterations are often hard to evaluate in detail when whole gut samples are used. Analyses based on pure ENS tissue can more effectively reflect the ongoing changes during pathological processes. Here, we present an optimized approach for the isolation of pure myenteric plexus (MP) from adult mouse and human. To do so, muscle tissue was individually digested with a purified collagenase. After incubation and a gentle mechanical disruption step, MP networks could be collected with anatomical integrity. These tissues could be stored and used either for immediate genomic, proteomic or in vitro approaches, and enteric neurospheres could be generated and differentiated. In a pilot experiment, the influence of bacterial lipopolysaccharide on human MP was analyzed using 2-dimensional gel electrophoresis. The method also allows investigation of factors that are secreted by myenteric tissue in vitro. The isolation of pure MP in large amounts allows new analytical approaches that can provide a new perspective in evaluating changes of the ENS in experimental models, human disease and aging.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.