ArticlePloS one2015
Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Association between lipid accumulation products and relative handgrip strength: a large population-based study.Scientific reports · 2025Article
- A lower atherogenic index of plasma was associated with a higher incidence of sarcopenia.Scientific reports · 2025Article
- Comparative proteomics and micro-RNA analysis of skeletal muscle cell small extracellular vesicles - Unique profiles in cells from severely obese individuals with type 2 diabetesFrontiers in physiology · 2025Article
- Altered Plasma Fatty Acid Abundance Is Associated with Cachexia in Treatment-Naïve Pancreatic Cancer.Cells · 2022Article
- Deprogram and reprogram to solve the riddle of insulin resistance.The Journal of clinical investigation · 2021Article
- Impaired glucose partitioning in primary myotubes from severely obese women with type 2 diabetes.American journal of physiology. Cell physiology · 2020Article
- Experimental Models of Sarcopenia: Bridging Molecular Mechanism and Therapeutic Strategy.Cells · 2020Review
- Altered mitochondrial network morphology and regulatory proteins in mitochondrial quality control in myotubes from severely obese humans with or without type 2 diabetes.Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme · 2020Article
- Higher lipid turnover and oxidation in cultured human myotubes from athletic versus sedentary young male subjects.Scientific reports · 2018Article
- Utilization of lactic acid in human myotubes and interplay with glucose and fatty acid metabolism.Scientific reports · 2018Article
- Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes.Journal of visualized experiments : JoVE · 2017Article
- Exercise in vivo marks human myotubes in vitro: Training-induced increase in lipid metabolism.PloS one · 2017Article
- Muscle Lipid Metabolism: Role of Lipid Droplets and Perilipins.Journal of diabetes research · 2017Review
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
About 80% of patients with type 2 diabetes are classified as overweight. However, only about 1/3 of severely obese subjects have type 2 diabetes. This indicates that several severely obese individuals may possess certain characteristics that protect them against type 2 diabetes. We therefore hypothesized that this apparent paradox could be related to fundamental differences in skeletal muscle lipid handling. Energy metabolism and metabolic flexibility were examined in human myotubes derived from severely obese subjects without (BMI 44±7 kg/m2) and with type 2 diabetes (BMI 43±6 kg/m2). Lower insulin sensitivity was observed in myotubes from severely obese subjects with type 2 diabetes. Lipolysis rate was higher, and oleic acid accumulation, triacylglycerol content, and fatty acid adaptability were lower in myotubes from severely obese subjects with type 2 diabetes compared to severely obese non-diabetic subjects. There were no differences in lipid distribution and mRNA and protein expression of the lipases HSL and ATGL, the lipase cofactor CGI-58, or the lipid droplet proteins PLIN2 and PLIN3. Glucose and oleic acid oxidation were also similar in cells from the two groups. In conclusion, myotubes established from severely obese donors with established type 2 diabetes had lower ability for lipid accumulation and higher lipolysis rate than myotubes from severely obese donors without diabetes. This indicates that a difference in intramyocellular lipid turnover might be fundamental in evolving type 2 diabetes.
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