ArticleZebrafish2015
Simple and rapid quantification of thrombocytes in zebrafish larvae.
Article in Zebrafish, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Deconstructing cardiovascular and coagulation-related traits links dietary ecology to multi-functional snake venom specificity.Evolution; international journal of organic evolution · 2026Article
- The viability and coagulation function of storing platelets can be maintained in plasma without cryoprotectants at -80 °C.Research and practice in thrombosis and haemostasis · 2026Article
- Assessing bleeding risk by thromboelastography when automated platelet count fails due to giant platelets.Blood vessels, thrombosis & hemostasis · 2026Article
- ER-associated degradation pathway protein SEL1L plays an evolutionarily conserved role in platelet adhesion.The Journal of clinical investigation · 2026Article
- Rbm8a deficiency causes hematopoietic defects by modulating Wnt/PCP signaling.Developmental biology · 2025Article
- Automated, high-throughput quantification of EGFP-expressing neutrophils in zebrafish by machine learning and a highly-parallelized microscope.bioRxiv : the preprint server for biology · 2023Article
- Article
- Genetic duplication of tissue factor reveals subfunctionalization in venous and arterial hemostasis.PLoS genetics · 2022Article
- Fishing for answers to hemostatic and thrombotic disease: Genome editing in zebrafish.Research and practice in thrombosis and haemostasis · 2022Review
- Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae.Scientific reports · 2021Article
- Deep Learning Approach for Quantification of Fluorescently Labeled Blood Cells inBioinformatics and biology insights · 2021Article
- A genetic modifier of venous thrombosis in zebrafish reveals a functional role for fibrinogen AαE in early hemostasis.Blood advances · 2020Article
- Analysis of factor V in zebrafish demonstrates minimal levels needed for early hemostasis.Blood advances · 2019Article
- Nfe2 is dispensable for early but required for adult thrombocyte formation and function in zebrafish.Blood advances · 2018Article
- Article
- Quantitative methods for studying hemostasis in zebrafish larvae.Methods in cell biology · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Platelets are a critical component of hemostasis, with disorders of number or function resulting in coagulation disturbances. Insights into these processes have primarily been realized through studies using mammalian models or tissues. Increasingly, zebrafish embryos and larvae have been used to study the protein and cellular components of hemostasis and thrombosis, including the thrombocyte, a nucleated platelet analog. However, investigations of thrombocytes have been somewhat limited due to lack of a robust and simple methodology for quantitation, an important component of platelet studies in mammals. Using video capture, we have devised an assay that produces a rapid, reproducible, and precise measurement of thrombocyte number in zebrafish larvae by counting fluorescently tagged cells. Averaging 1000 frames, we were able to subtract background fluorescence, thus limiting assessment to circulating thrombocytes. This method facilitated rapid assessment of relative thrombocyte counts in a population of 372 zebrafish larvae by a single operator in less than 3 days. This technique requires basic microscopy equipment and rudimentary programming, lends itself to high throughput analysis, and will enhance future studies of thrombopoiesis in the zebrafish.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.