Evidence map›Paper›PMID 25789886›Full record

ArticleJournal of the American Chemical Society2015

Integrating epigenetic modulators into NanoScript for enhanced chondrogenesis of stem cells.

Sahishnu Patel, Thanapat Pongkulapa, Perry T Yin, Ganesh N Pandian, Christopher Rathnam, Toshikazu Bando, Thangavel Vaijayanthi, Hiroshi Sugiyama, Ki-Bum Lee

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Chemical Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 27 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Sahishnu Patel†Department of Chemistry and Chemical Biology and ‡Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854-8087, United States.
Thanapat Pongkulapa†Department of Chemistry and Chemical Biology and ‡Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854-8087, United States.
Perry T Yin†Department of Chemistry and Chemical Biology and ‡Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854-8087, United States.
Ganesh N Pandian†Department of Chemistry and Chemical Biology and ‡Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854-8087, United States.
Christopher Rathnam†Department of Chemistry and Chemical Biology and ‡Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854-8087, United States.
Toshikazu Bando†Department of Chemistry and Chemical Biology and ‡Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854-8087, United States.
Thangavel Vaijayanthi†Department of Chemistry and Chemical Biology and ‡Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854-8087, United States.
Hiroshi Sugiyama†Department of Chemistry and Chemical Biology and ‡Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854-8087, United States.
Ki-Bum Lee†Department of Chemistry and Chemical Biology and ‡Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854-8087, United States.
Rutgers, The State University of New Jersey · USKyoto University · JP

Funding

Combinatorial approaches for studying multiple cues regulating human pluripotentDP2OD006462 · OD · RUTGERS, THE STATE UNIV OF N.J. · PI LEE, KIBUM · 2009 to 2009
$2.3M
Novel magnetic core/shell nanoparticle-based stem cell therapy to direct neural sR21NS085569 · NINDS · RUTGERS, THE STATE UNIV OF N.J. · PI LEE, KIBUM · 2013 to 2014
$421k
NCCDPHP CDC HHS 1DP20D006462-01NIH HHS DP2 OD006462NINDS NIH HHS 1R21NS085569-01NINDS NIH HHS R21 NS085569
6 · The paper itself

Abstract

N-(4-Chloro-3-(trifluoromethyl)phenyl)-2-ethoxybenzamide (CTB) is a small molecule that functions by altering the chromatin architecture to modulate gene expression. We report a new CTB derivative with increased solubility and demonstrate CTB's functionality by conjugating it on the recently established NanoScript platform to enhance gene expression and induce stem cell differentiation. NanoScript is a nanoparticle-based artificial transcription factor that emulates the structure and function of transcription factor proteins (TFs) to effectively regulate endogenous gene expression. Modifying NanoScript with CTB will more closely replicate the TF structure and enhance CTB functionality and gene expression. To this end, we first conjugated CTB onto NanoScript and initiated a time-dependent increase in histone acetyltransferase activity. Next, because CTB is known to trigger the pathway involved in regulating Sox9, a master regulator of chondrogenic differentiation, we modifed a Sox9-specific NanoScript with CTB to enhance chondrogenic gene activity and differentiation. Because NanoScript is a tunable and robust platform, it has potential for various gene-regulating applications, such as stem cell differentiation.

Indexed as

BenzamidesBiomimetic MaterialsChondrogenesisEpigenesis, GeneticMesenchymal Stem CellsNanoparticlesTranscription FactorsBenzamidesTranscription Factors

Identifiers

PMID25789886
PMCPMC5702886
OpenAlexW1992880689

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.