ArticleJundishapur journal of microbiology2015
Molecular Genotyping of Shigella sonnei Strains Isolated From Children With Bloody Diarrhea Using Pulsed Field Gel Electrophoresis on the Total Genome and PCR-RFLP of IpaH and IpaBCD Genes.
Article in Jundishapur journal of microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 18 citations in OpenAlex.
- Article
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- DNA sequencing, genomes and genetic markers of microbes on fruits and vegetables.Microbial biotechnology · 2021Review
- High Rates of Antimicrobial Resistance and Virulence Gene Distribution AmongInfection and drug resistance · 2020Article
- Preharvest Transmission Routes of Fresh Produce Associated Bacterial Pathogens with Outbreak Potentials: A Review.International journal of environmental research and public health · 2019Review
- Bacterial CRISPR Regions: General Features and their Potential for Epidemiological Molecular Typing Studies.The open microbiology journal · 2018Review
- Profiling of Virulence-associated Factors inOsong public health and research perspectives · 2017Article
- Diversity of Variable Number Tandem Repeat Loci in Shigella Species Isolated from Pediatric Patients.International journal of molecular and cellular medicine · 2015Article
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3 authors at 2 institutions in 1 country.
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Abstract
backgroundIdentification, understanding of antibiotic sensitivity patterns and molecular characterization of genetic elements of Shigella species are important because of both epidemiological and clinical indications in developing countries.
objectivesThe aim of this study was to analyze molecular epidemiology of Shigella isolates recovered from children with diarrhea in Shiraz (Southern Iran), using IpaH and IpaBCD PCR-restriction fragment length polymorphism (RFLP), and to determine pulsed field gel electrophoresis (PFGE) patterns of total DNA of the S. sonnei isolates to find the clonality among these strains. PATIENTS AND
methodsA total of 82 clinical strains of Shigella spp., S. sonnei (n = 61), S. flexneri (n = 16), Shigella boydii (n = 3) and S. dysenteriae (n = 2) isolated from the stool samples of 719 patients, aged two months to 14 years, with positive occult blood (OB) test were characterized based on their IpaH and IpaBCD genes PCR-RFLP patterns. Genomic DNAs of S. sonnei strains were analyzed by PFGE.
resultsAll Shigella isolates were positive for both invasive genes and showed homogeneous profiles for such genes except for two S. sonnei strains, which had IpaH bands with different sizes and PCR-RFLP profiles. Forty palsotypes were determined among the 41 S. sonnei strains. Sample patterns were divided into two groups based on the drawn dendrogram with a similarity range of 70% to 100%.
conclusionsThe results revealed that the strains under study could be epidemically related. It seems that an alternative subtyping method is needed to study the relationship among clinical S. sonnei strains and their transmission. Here, we reported for the first time, two strains of S. sonnei with a different PCR-RFLP pattern for IpaH gene.
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