ArticleBiotechnology for biofuels2015
The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
116 citing papers in PubMed, 1 synthesis or guideline pooled it, 367 citations in OpenAlex.
- Pooled it
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- Poly(DADMAC) incorporated lipid nanoparticles enhance the delivery of antimicrobial peptides into plant cells.Scientific reports · 2026Article
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- Effect of niosome formation with chitosan coating on the stability and absorption of orally administered vesicular ursolic acid.Journal of materials science. Materials in medicine · 2026Article
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- Characterization of nanoplastics and small-sized microplastics in sewage treatment.Scientific reports · 2025Article
- The TRPM5 Antagonist Triphenylphosphine Oxide Increases Sebaceous Lipogenesis and Modulates Immune Phenotype of Human Sebocytes in a TRPM5-Independent Manner.Experimental dermatology · 2025Article
- Sulfo-phospho-vanillin method for screening Aurantiochytrium strains with high docosahexaenoic acid levels.AMB Express · 2025Article
- Mechanism of nanomaterial-induced lipid droplet formation inFrontiers in plant science · 2025Article
- Adaptive Synthesis, Supramolecular Behavior, and Biological Properties of Amphiphilic Carbosilane-Phosphonium Dendrons with Tunable Structure.Biomacromolecules · 2024Article
- Polyamines mediate cellular energetics and lipid metabolism through mitochondrial respiration to facilitate virus replication.PLoS pathogens · 2024Article
- Co-cultivation of microalgae and bacteria for optimal bioenergy feedstock production in wastewater by using response surface methodology.Scientific reports · 2024Article
- Three-Dimensional Imaging of Bioinspired Lipidic Mesophases Using Multicolored Light-Emitting Carbon Nanodots.The journal of physical chemistry letters · 2024Article
- Fluorescent Chiral Quantum Dots to Unveil Origin-Dependent Exosome Uptake and Cargo Release.ACS applied bio materials · 2024Article
56 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. Microalgae constitute a diverse group of microorganisms with advantages like fast and efficient growth. In addition, they do not compete for arable land and offer very high lipid yield potential. Major challenges for the development of this resource are to select lipid-rich strains using high-throughput staining for neutral lipid content in microalgae species. For this purpose, the fluorescent dyes most commonly used to quantify lipids are Nile red and BODIPY 505/515. Their fluorescent staining for lipids offers a rapid and inexpensive analysis tool to measure neutral lipid content, avoiding time-consuming and costly gravimetric analysis. This review collates and presents recent advances in algal lipid staining and focuses on Nile red and BODIPY 505/515 staining characteristics. The available literature addresses the limitations of fluorescent dyes under certain conditions, such as spectral properties, dye concentrations, cell concentrations, temperature and incubation duration. Moreover, the overall conclusion of the present review study gives limitations on the use of fluorochrome for screening of lipid-rich microalgae species and suggests improved protocols for staining recalcitrant microalgae and recommendations for the staining quantification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.