ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2015
Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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Who cites it
54 citing papers in PubMed, 85 citations in OpenAlex.
- Pericytes as key mediators of microenvironmental signaling crosstalk: implications in vascular diseases and cancer.Frontiers in cell and developmental biology · 2026Review
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- Longitudinal Investigation of Brain and Spinal Cord Pericytes After Inducible PDGFRβJournal of neurochemistry · 2025Article
- The molecular landscape of vascular cells in the human brain.Nature reviews. Cardiology · 2024Article
- Profiling human brain vascular cells using single-cell transcriptomics and organoids.Nature protocols · 2024Review
- Review
- CD13 facilitates immune cell migration and aggravates acute injury but promotes chronic post-stroke recovery.Journal of neuroinflammation · 2023Article
- Review
- Disentangling brain vasculature in neurogenesis and neurodegeneration using single-cell transcriptomics.Trends in neurosciences · 2023Review
- Connexin 43-mediated neurovascular interactions regulate neurogenesis in the adult brain subventricular zone.Cell reports · 2023Article
- Outcomes of the 2019 hydrocephalus association workshop, "Driving common pathways: extending insights from posthemorrhagic hydrocephalus".Fluids and barriers of the CNS · 2023Review
- Brain pericyte biology: from physiopathological mechanisms to potential therapeutic applications in ischemic stroke.Frontiers in cellular neuroscience · 2023Review
- Article
- Review
- The Role of Stem Cells in the Therapy of Stroke.Current neuropharmacology · 2022Review
- Assessing the Role of Ependymal and Vascular Cells as Sources of Extracellular Cues Regulating the Mouse Ventricular-Subventricular Zone Neurogenic Niche.Frontiers in cell and developmental biology · 2022Review
- ErbB3 is a critical regulator of cytoskeletal dynamics in brain microvascular endothelial cells: Implications for vascular remodeling and blood brain barrier modulation.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2021Article
- Neural crest cell-derived pericytes act as pro-angiogenic cells in human neocortex development and gliomas.Fluids and barriers of the CNS · 2021Review
- Mural Cells: Potential Therapeutic Targets to Bridge Cardiovascular Disease and Neurodegeneration.Cells · 2021Review
- Bioengineering the neurovascular niche to study the interaction of neural stem cells and endothelial cells.APL bioengineering · 2021Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
Abstract
Adult neural stem cells reside in specialized niches. In the ventricular-subventricular zone (V-SVZ), quiescent neural stem cells (qNSCs) become activated (aNSCs), and generate transit amplifying cells (TACs), which give rise to neuroblasts that migrate to the olfactory bulb. The vasculature is an important component of the adult neural stem cell niche, but whether vascular cells in neurogenic areas are intrinsically different from those elsewhere in the brain is unknown. Moreover, the contribution of pericytes to the neural stem cell niche has not been defined. Here, we describe a rapid FACS purification strategy to simultaneously isolate primary endothelial cells and pericytes from brain microregions of nontransgenic mice using CD31 and CD13 as surface markers. We compared the effect of purified vascular cells from a neurogenic (V-SVZ) and non-neurogenic brain region (cortex) on the V-SVZ stem cell lineage in vitro. Endothelial and pericyte diffusible signals from both regions differentially promote the proliferation and neuronal differentiation of qNSCs, aNSCs, and TACs. Unexpectedly, diffusible cortical signals had the most potent effects on V-SVZ proliferation and neurogenesis, highlighting the intrinsic capacity of non-neurogenic vasculature to support stem cell behavior. Finally, we identify PlGF-2 as an endothelial-derived mitogen that promotes V-SVZ cell proliferation. This purification strategy provides a platform to define the functional and molecular contribution of vascular cells to stem cell niches and other brain regions under different physiological and pathological states.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.