ArticleJournal of the American Society of Nephrology : JASN2015
Store-Operated Ca2+ Channels in Mesangial Cells Inhibit Matrix Protein Expression.
Article in Journal of the American Society of Nephrology : JASN, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 49 citations in OpenAlex.
- The CaPediatric nephrology (Berlin, Germany) · 2025Review
- I-mfa, Mesangial Cell TRPC1 Channel, and Regulation of GFR.Journal of the American Society of Nephrology : JASN · 2025Article
- Article
- PAR1-mediated Non-periodical Synchronized Calcium Oscillations in Human Mesangial Cells.Function (Oxford, England) · 2024Article
- Clinical and pathological significance of Orai1 channel expression in human diabetic nephropathy.Kidney research and clinical practice · 2024Article
- Calcium signalling and transport in the kidney.Nature reviews. Nephrology · 2024Review
- Non-voltage-gated CaAmerican journal of physiology. Renal physiology · 2024Review
- Store-operated CaExperimental biology and medicine (Maywood, N.J.) · 2023Review
- Article
- Nanomedicines for the management of diabetic nephropathy: present progress and prospects.Frontiers in endocrinology · 2023Review
- Ion channels and channelopathies in glomeruli.Physiological reviews · 2023Review
- Pathophysiology of mesangial expansion in diabetic nephropathy: mesangial structure, glomerular biomechanics, and biochemical signaling and regulation.Journal of biological engineering · 2022Review
- Enhanced Orai1-mediated store-operated CaThe Journal of biological chemistry · 2022Article
- Ion channels as a therapeutic target for renal fibrosis.Frontiers in physiology · 2022Review
- Insulin-activated store-operated CaNature communications · 2021Article
- Annexin A1 alleviates kidney injury by promoting the resolution of inflammation in diabetic nephropathy.Kidney international · 2021Article
- Store-operated calcium entry: Pivotal roles in renal physiology and pathophysiology.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- SHROOM3, the gene associated with chronic kidney disease, affects the podocyte structure.Scientific reports · 2020Article
- Stimulation of ORAI1 expression, store-operated CaPflugers Archiv : European journal of physiology · 2020Article
- Inhibition of interleukin-6 on matrix protein production by glomerular mesangial cells and the pathway involved.American journal of physiology. Renal physiology · 2020Article
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
Abstract
Accumulation of extracellular matrix derived from glomerular mesangial cells is an early feature of diabetic nephropathy. Ca(2+) signals mediated by store-operated Ca(2+) channels regulate protein production in a variety of cell types. The aim of this study was to determine the effect of store-operated Ca(2+) channels in mesangial cells on extracellular matrix protein expression. In cultured human mesangial cells, activation of store-operated Ca(2+) channels by thapsigargin significantly decreased fibronectin protein expression and collagen IV mRNA expression in a dose-dependent manner. Conversely, inhibition of the channels by 2-aminoethyl diphenylborinate significantly increased the expression of fibronectin and collagen IV. Similarly, overexpression of stromal interacting molecule 1 reduced, but knockdown of calcium release-activated calcium channel protein 1 (Orai1) increased fibronectin protein expression. Furthermore, 2-aminoethyl diphenylborinate significantly augmented angiotensin II-induced fibronectin protein expression, whereas thapsigargin abrogated high glucose- and TGF-β1-stimulated matrix protein expression. In vivo knockdown of Orai1 in mesangial cells of mice using a targeted nanoparticle siRNA delivery system resulted in increased expression of glomerular fibronectin and collagen IV, and mice showed significant mesangial expansion compared with controls. Similarly, in vivo knockdown of stromal interacting molecule 1 in mesangial cells by recombinant adeno-associated virus-encoded shRNA markedly increased collagen IV protein expression in renal cortex and caused mesangial expansion in rats. These results suggest that store-operated Ca(2+) channels in mesangial cells negatively regulate extracellular matrix protein expression in the kidney, which may serve as an endogenous renoprotective mechanism in diabetes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.