ArticleScientific reports2015
Single cell visualization of transcription kinetics variance of highly mobile identical genes using 3D nanoimaging.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Transcription dynamics and genome organization in the mammalian nucleus: Recent advances.Molecular cell · 2025Review
- Transcription-dependent mobility of single genes and genome-wide motions in live human cells.Nature communications · 2024Article
- The intracellular visualization of exogenous DNA in fluorescence microscopy.Drug delivery and translational research · 2024Review
- Phase separation in transcription factor dynamics and chromatin organization.Current opinion in structural biology · 2021Review
- RNA polymerase II speed: a key player in controlling and adapting transcriptome composition.The EMBO journal · 2021Review
- Mechanical Regulation of Transcription: Recent Advances.Trends in cell biology · 2021Review
- Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility.Molecular cell · 2019Article
- Live-cell imaging reveals the interplay between transcription factors, nucleosomes, and bursting.The EMBO journal · 2019Article
- Measuring Mobility in Chromatin by Intensity-Sorted FCS.Biophysical journal · 2019Article
- Gene transcription in bursting: a unified mode for realizing accuracy and stochasticity.Biological reviews of the Cambridge Philosophical Society · 2019Article
- Label-free Multiscale Transport Imaging of the Living Cell.Biophysical journal · 2018Article
- Estrogen-dependent control and cell-to-cell variability of transcriptional bursting.Molecular systems biology · 2018Article
- Real-Time Imaging of a Single Gene Reveals Transcription-Initiated Local Confinement.Biophysical journal · 2017Article
- Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape.Scientific reports · 2017Article
- Mechanobiology of Chromatin and the Nuclear Interior.Cellular and molecular bioengineering · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Multi-cell biochemical assays and single cell fluorescence measurements revealed that the elongation rate of Polymerase II (PolII) in eukaryotes varies largely across different cell types and genes. However, there is not yet a consensus whether intrinsic factors such as the position, local mobility or the engagement by an active molecular mechanism of a genetic locus could be the determinants of the observed heterogeneity. Here by employing high-speed 3D fluorescence nanoimaging techniques we resolve and track at the single cell level multiple, distinct regions of mRNA synthesis within the model system of a large transgene array. We demonstrate that these regions are active transcription sites that release mRNA molecules in the nucleoplasm. Using fluctuation spectroscopy and the phasor analysis approach we were able to extract the local PolII elongation rate at each site as a function of time. We measured a four-fold variation in the average elongation between identical copies of the same gene measured simultaneously within the same cell, demonstrating a correlation between local transcription kinetics and the movement of the transcription site. Together these observations demonstrate that local factors, such as chromatin local mobility and the microenvironment of the transcription site, are an important source of transcription kinetics variability.
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Registered trials
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