ArticleMolecular & cellular proteomics : MCP2015
Optimized Analytical Procedures for the Untargeted Metabolomic Profiling of Human Urine and Plasma by Combining Hydrophilic Interaction (HILIC) and Reverse-Phase Liquid Chromatography (RPLC)-Mass Spectrometry.
Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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Who cites it
98 citing papers in PubMed.
- COVID-19 convalescent plasma composition and immunological effects in severe patients.Journal of autoimmunity · 2021Trial
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- UHPLC-Q/TOF-MS Characterization of Danshen-Chuanxiong and Mechanistic Insights Into Its Protective Effects Against AβJournal of separation science · 2026Article
- Exploring the Metabolic Impact of Traumatic Brain Injury in CCI Mouse Models: A Focus on Early and Prolonged Injury Responses.International journal of molecular sciences · 2026Article
- Low-Temperature HILIC Provides Enhanced Separations and Stability for LC-MS-Based Metabolomics.Journal of proteome research · 2026Article
- Practical Workflow for Building Local Mass Spectral Libraries for Untargeted Metabolomics.Metabolites · 2026Article
- A Practical Framework for Wastewater-Based Monitoring of Substance Use in Public Health Settings.International journal of environmental research and public health · 2026Article
- Novel amino acid and fatty acid signatures linked to type 2 diabetes risk after gestational diabetes mellitus.Cardiovascular diabetology · 2026Article
- Untargeted mass spectrometry to investigate ocean acidification inThe Analyst · 2026Article
- Investigating Variability in Metabolomics: A Comparative Study of Analytical Platforms and Blood Matrices Using HPLC-HRMS.Molecules (Basel, Switzerland) · 2026Article
- An encyclopedia of the cord blood metabolome reveals maternal-fetal interactions and disease risk.Cell reports. Medicine · 2026Article
- Mapping the unknown: an anchor-based molecular networking workflow for comprehensive identification of new psychoactive substances and analogues.Analytical and bioanalytical chemistry · 2026Article
- Systemic elevation of gut derivedFrontiers in physiology · 2026Article
- A Nonketotic Hyperglycinemia Mouse Shows Wide-Ranging Biochemical Consequences of Elevated Glycine, Reduced Folate One-Carbon Charging, and Serine Deficiency.Journal of inherited metabolic disease · 2026Article
- Maternal lipidomic signatures of preterm and small-for-gestational-age newborn infants in low- and middle-income countries.Science advances · 2025Article
- Multiclass Assays for Measuring Environmental Chemical Mixture Exposure: Analytical Methodologies and Applications in Exposomics Research.Metabolites · 2025Review
- Investigating stage-specific metabolic alterations in colorectal cancer through urine metabolomics.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Glia-derived noncanonical fatty acid binding protein modulates brain lipid storage and clearance.Science advances · 2025Article
- Oxidative stress is a shared characteristic of ME/CFS and Long COVID.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
38 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
Abstract
Profiling of body fluids is crucial for monitoring and discovering metabolic markers of health and disease and for providing insights into human physiology. Since human urine and plasma each contain an extreme diversity of metabolites, a single liquid chromatographic system when coupled to mass spectrometry (MS) is not sufficient to achieve reasonable metabolome coverage. Hydrophilic interaction liquid chromatography (HILIC) offers complementary information to reverse-phase liquid chromatography (RPLC) by retaining polar metabolites. With the objective of finding the optimal combined chromatographic solution to profile urine and plasma, we systematically investigated the performance of five HILIC columns with different chemistries operated at three different pH (acidic, neutral, basic) and five C18-silica RPLC columns. The zwitterionic column ZIC-HILIC operated at neutral pH provided optimal performance on a large set of hydrophilic metabolites. The RPLC columns Hypersil GOLD and Zorbax SB aq were proven to be best suited for the metabolic profiling of urine and plasma, respectively. Importantly, the optimized HILIC-MS method showed excellent intrabatch peak area reproducibility (CV < 12%) and good long-term interbatch (40 days) peak area reproducibility (CV < 22%) that were similar to those of RPLC-MS procedures. Finally, combining the optimal HILIC- and RPLC-MS approaches greatly expanded metabolome coverage with 44% and 108% new metabolic features detected compared with RPLC-MS alone for urine and plasma, respectively. The proposed combined LC-MS approaches improve the comprehensiveness of global metabolic profiling of body fluids and thus are valuable for monitoring and discovering metabolic changes associated with health and disease in clinical research studies.
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