ArticleProceedings of the National Academy of Sciences of the United States of America2015
Ribosomal frameshifting and dual-target antiactivation restrict quorum-sensing-activated transfer of a mobile genetic element.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Quorum Sensing Regulator CinR Directly Activates the Catalase-Peroxidase GeneAntioxidants (Basel, Switzerland) · 2026Article
- Structural basis for control of integrative and conjugative element excision and transfer by the oligomeric winged helix-turn-helix protein RdfS.Nucleic acids research · 2025Article
- DUF2285 is a novel helix-turn-helix domain variant that orchestrates both activation and antiactivation of conjugative element transfer in proteobacteria.Nucleic acids research · 2023Article
- Article
- Crystallographic and X-ray scattering study of RdfS, a recombination directionality factor from an integrative and conjugative element.Acta crystallographica. Section D, Structural biology · 2022Article
- An epigenetic switch activates bacterial quorum sensing and horizontal transfer of an integrative and conjugative element.Nucleic acids research · 2022Article
- Why are rhizobial symbiosis genes mobile?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Comparative analysis of integrative and conjugative mobile genetic elements in the genusMicrobial genomics · 2021Article
- Rhizobium leguminosarum bv. trifolii NodD2 Enhances Competitive Nodule Colonization in the Clover-Rhizobium Symbiosis.Applied and environmental microbiology · 2020Article
- Symbiosis islands of Loteae-nodulatingMicrobial genomics · 2020Article
- Review
- Regulation Mediated by N-Acyl Homoserine Lactone Quorum Sensing Signals in the Rhizobium-Legume Symbiosis.Genes · 2018Review
- Rhizobia: from saprophytes to endosymbionts.Nature reviews. Microbiology · 2018Review
- Sequential induction of three recombination directionality factors directs assembly of tripartite integrative and conjugative elements.PLoS genetics · 2018Article
- The hidden life of integrative and conjugative elements.FEMS microbiology reviews · 2017Review
- Plant nodulation inducers enhance horizontal gene transfer of Azorhizobium caulinodans symbiosis island.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Assembly and transfer of tripartite integrative and conjugative genetic elements.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Ribosomal frameshifting and transcriptional slippage: From genetic steganography and cryptography to adventitious use.Nucleic acids research · 2016Review
- HIV-1 and Human PEG10 Frameshift Elements Are Functionally Distinct and Distinguished by Novel Small Molecule Modulators.PloS one · 2015Article
- Silencing quorum sensing and ICE mobility through antiactivation and ribosomal frameshifting.Mobile genetic elementsArticle
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Symbiosis islands are integrative and conjugative mobile genetic elements that convert nonsymbiotic rhizobia into nitrogen-fixing symbionts of leguminous plants. Excision of the Mesorhizobium loti symbiosis island ICEMlSym(R7A) is indirectly activated by quorum sensing through TraR-dependent activation of the excisionase gene rdfS. Here we show that a +1 programmed ribosomal frameshift (PRF) fuses the coding sequences of two TraR-activated genes, msi172 and msi171, producing an activator of rdfS expression named Frameshifted excision activator (FseA). Mass-spectrometry and mutational analyses indicated that the PRF occurred through +1 slippage of the tRNA(phe) from UUU to UUC within a conserved msi172-encoded motif. FseA activated rdfS expression in the absence of ICEMlSym(R7A), suggesting that it directly activated rdfS transcription, despite being unrelated to any characterized DNA-binding proteins. Bacterial two-hybrid and gene-reporter assays demonstrated that FseA was also bound and inhibited by the ICEMlSym(R7A)-encoded quorum-sensing antiactivator QseM. Thus, activation of ICEMlSym(R7A) excision is counteracted by TraR antiactivation, ribosomal frameshifting, and FseA antiactivation. This robust suppression likely dampens the inherent biological noise present in the quorum-sensing autoinduction circuit and ensures that ICEMlSym(R7A) transfer only occurs in a subpopulation of cells in which both qseM expression is repressed and FseA is translated. The architecture of the ICEMlSym(R7A) transfer regulatory system provides an example of how a set of modular components have assembled through evolution to form a robust genetic toggle that regulates gene transcription and translation at both single-cell and cell-population levels.
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