ArticleMicrobes and environments2015
A Comprehensive, Automatically Updated Fungal ITS Sequence Dataset for Reference-Based Chimera Control in Environmental Sequencing Efforts.
Article in Microbes and environments, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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106 citing papers in PubMed.
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- Optimization of a magnetic bead-based DNA extraction method combined with 12S rRNA barcoding for species traceability in fish oil products.Food chemistry. Molecular sciences · 2026Article
- Global dataset of soil eukaryotic communities created with a uniform protocol and long read sequencing.Scientific data · 2026Article
- Sampling Design and Sample Processing Affect Soil Biodiversity Assessments.Molecular ecology resources · 2026Article
- Two decades of mucormycosis: molecular epidemiology and antifungal susceptibility trends in Türkiye.Frontiers in cellular and infection microbiology · 2026Article
- Lignin enrichment selects specialized fungal communities from contrasting soils.Sustainable microbiology · 2026Article
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- Comparison of QIIME1 and QIIME2 for Analyzing Fungal Samples from Various Built Environments.Microorganisms · 2025Article
- Environmental Yeast Abundance and Diversity Assessment in Recreation Areas of Bangkok, Thailand.Environmental microbiology reports · 2025Article
- Teknonaturalist: A Snakemake Pipeline for Assessing Fungal Diversity From Plant Genome Bycatch.Molecular ecology resources · 2025Article
- Protein Coding Low-CopyEcology and evolution · 2025Article
- Dynamics and Insights into the Unique Ecological Guild of Fungi in Bacteria-Bioaugmented Anaerobic Digesters.Journal of fungi (Basel, Switzerland) · 2025Article
- Clonal integration and Bacillus subtilis modulate Glechoma longituba performance and soil microbial communities.PloS one · 2025Article
- The effects of clonal integration and earthworms on the growth, active constituent accumulation inFrontiers in plant science · 2025Article
- Fungal and bacterial pathogenic co-infections mainly lead to the assembly of microbial community in tobacco stems.Open life sciences · 2025Article
46 more citing papers are in PubMed but not listed here.
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Authors and funding
16 authors.
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Abstract
The nuclear ribosomal internal transcribed spacer (ITS) region is the most commonly chosen genetic marker for the molecular identification of fungi in environmental sequencing and molecular ecology studies. Several analytical issues complicate such efforts, one of which is the formation of chimeric-artificially joined-DNA sequences during PCR amplification or sequence assembly. Several software tools are currently available for chimera detection, but rely to various degrees on the presence of a chimera-free reference dataset for optimal performance. However, no such dataset is available for use with the fungal ITS region. This study introduces a comprehensive, automatically updated reference dataset for fungal ITS sequences based on the UNITE database for the molecular identification of fungi. This dataset supports chimera detection throughout the fungal kingdom and for full-length ITS sequences as well as partial (ITS1 or ITS2 only) datasets. The performance of the dataset on a large set of artificial chimeras was above 99.5%, and we subsequently used the dataset to remove nearly 1,000 compromised fungal ITS sequences from public circulation. The dataset is available at http://unite.ut.ee/repository.php and is subject to web-based third-party curation.
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