Evidence map›Paper›PMID 25786869›Full record

ArticleGene therapy2015

Intravaginal HPV DNA vaccination with electroporation induces local CD8+ T-cell immune responses and antitumor effects against cervicovaginal tumors.

Y Sun, S Peng, J Qiu, J Miao, B Yang, J Jeang, C-F Hung, T-C Wu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gene therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 27 citations in OpenAlex.

  1. Trial
  2. Review
  3. Article
  4. Review
  5. Review
  6. Article
  7. Control of Tumors by Antigen-Specific CD8Journal of immunology research · 2022
    Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Review
  17. Novel delivery approaches for cancer therapeutics.Journal of controlled release : official journal of the Controlled Release Society · 2015
    Review
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Y Sun1] Department of Obstetrics and Gynecology, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, China [2] Departments of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA.
S PengDepartments of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA.
J Qiu1] Departments of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA [2] Department of Obstetrics and Gynecology, Shanghai Tenth People's Hospital of Tongji University Shanghai, Shanghai, China.
J Miao1] Departments of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA [2] Department of General Surgery, The Second Hospital of Dalian Medical University, Dalian, Liaoning, China [3] Department of Hepatobiliary and Pancreatic Surgery, The Second Hospital of Dalian Medical University, Dalian, Liaoning, China.
B YangDepartments of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA.
J JeangDepartments of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA.
C-F Hung1] Departments of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA [2] Department of Oncology, Johns Hopkins Medical Institutions, Baltimore, MD, USA.
T-C Wu1] Departments of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA [2] Department of Oncology, Johns Hopkins Medical Institutions, Baltimore, MD, USA [3] Department of Obstetrics and Gynecology, Johns Hopkins Medical Institutions, Baltimore, MD, USA [4] Department of Molecular Microbiology and Immunology, Johns Hopkins Medical Institutions, Baltimore, MD, USA.
Johns Hopkins University · USShanghai Jiao Tong University · CN

Funding

Treatment of HIV- and HIV+ Patients with HPV16+ CIN2/3 Using pNGLV4a-hCRTE6E7L2 DNA vaccine administered intramuscularly via electroporationP50CA098252 · NCI · JOHNS HOPKINS UNIVERSITY · PI WARNER KING HUH, TZYY-CHOOU WU · 2003 to 2026
$53.5M
ENHANCING HPV-16 E6-SPECIFIC ANTITUMOR IMMUNITYR01CA114425 · NCI · JOHNS HOPKINS UNIVERSITY · PI WU, TZYY-CHOOU · 2005 to 2016
$3.0M
Research Pilot Project 2P20CA192988 · NCI · JOHNS HOPKINS UNIVERSITY · PI WU, TZYY-CHOOU · 2014 to 2017
$702k
Upregulation of Nanog as an Innovative Mechanism for Cancer Drug Resistance.R21CA178255 · NCI · JOHNS HOPKINS UNIVERSITY · PI WU, TZYY-CHOOU · 2014 to 2015
$388k
NCI NIH HHS 2R01CA114425-06NCI NIH HHS 5R21CA178255-02NCI NIH HHS P20 CA192988NCI NIH HHS P50 CA098252NCI NIH HHS P50CA098252NCI NIH HHS R01 CA114425NCI NIH HHS R21 CA178255
6 · The paper itself

Abstract

Therapeutic human papillomavirus (HPV) vaccines have the potential to inhibit the progression of an established HPV infection to precancer and cancer lesions by targeting HPV oncoproteins. We have previously developed a therapeutic DNA vaccine encoding calreticulin (CRT) linked to E7, CRT/E7 DNA vaccine, for use in the treatment of HPV-associated lesions. Since the transfection efficiency of DNA vaccines administered in vivo is typically low, we examined the use of electroporation as well as different routes of administration to enhance antigen-specific tumor control. We tested the effects of the CRT/E7 DNA vaccine administered intramuscularly or intravaginally, with or without electroporation, on the generation of CD8+ T-cell immunity and therapeutic antitumor effects in HPV16 E7-expressing cervicovaginal tumor-bearing mice. We found that intravaginal vaccination of CRT/E7 DNA followed by electroporation-induced potent E7-specific CD8(+) T-cell responses in the cervicovaginal tract, compared with intramuscular injection followed by electroporation. Furthermore, tumor-bearing mice vaccinated intravaginally followed by electroporation had an enhanced survival, antitumor effects and local production of IFN-γ+CD8+ T cells compared with those vaccinated intramuscularly with electroporation. Thus, we show that intravaginal CRT/E7 DNA vaccination followed by electroporation generates the most potent therapeutic antitumor effects against an orthotopic E7-expressing tumor model. The current study will have significant clinical implications once a clinically applicable electroporation device for intravaginal use becomes available.

Indexed as

Administration, IntravaginalAnimalsAntineoplastic AgentsCD8-Positive T-LymphocytesCell Line, TumorElectroporationFemaleMice, Inbred C57BLPapillomavirus VaccinesUterine Cervical NeoplasmsVaccines, DNAAntineoplastic AgentsPapillomavirus VaccinesVaccines, DNA

Identifiers

PMID25786869
PMCPMC4490060
OpenAlexW1970289640

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.