ArticlePlant cell reports2015
A highly efficient maize nucellus protoplast system for transient gene expression and studying programmed cell death-related processes.
Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 49 citations in OpenAlex.
- Screening and functional identification of CtMYB2 as a positive regulator of flavonoid biosynthesis in safflower (Carthamus tinctorius L.).BMC plant biology · 2026Article
- Establishment of protoplast isolation, purification and transient transformation system from Rhododendron petals.Protoplasma · 2026Article
- Protoplast-Based Regeneration Enables CRISPR/Cas9 Application in Two TemperatePlants (Basel, Switzerland) · 2025Article
- CtMYB1 regulates flavonoid biosynthesis in safflower flower by binding the CAACCA elements.PloS one · 2025Article
- Optimization of preparation and transformation of protoplasts from Populus simonii × P. nigra leaves and subcellular localization of the major latex protein 328 (MLP328).Plant methods · 2024Article
- A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze].Plant methods · 2023Article
- Identification and Characterization of CtUGT3 as the Key Player of Astragalin Biosynthesis inJournal of agricultural and food chemistry · 2023Article
- Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera.Plant methods · 2022Article
- Programmed Cell Death in DevelopingInternational journal of molecular sciences · 2021Article
- An efficient sorghum protoplast assay for transient gene expression and gene editing by CRISPR/Cas9.PeerJ · 2020Article
- Optimization of isolation and transfection conditions of maize endosperm protoplasts.Plant methods · 2020Article
- Plant proteases during developmental programmed cell death.Journal of experimental botany · 2019Review
- Optimized Methods for the Isolation ofMolecules and cells · 2016Article
- Development of a rapid, low-cost protoplast transfection system for switchgrass (Panicum virgatum L.).Plant cell reports · 2016Article
- Functional characterization and reconstitution of ABA signaling components using transient gene expression in rice protoplasts.Frontiers in plant science · 2015Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageConditions for the isolation and transfection of maize nucellus protoplasts were established. We demonstrated its utilization for protein expression, localization, protein-protein interaction, and the investigation of PCD-related processes. Plant protoplasts are an important and versatile cell system that is widely used in the analysis of gene characterization and diverse signaling pathways. Programmed cell death (PCD) occurs throughout the life of plants from embryogenesis to fertilization. The maize nucellus undergoes typical PCD during development of the embryo sac. The nucellus protoplast shows potential for use in research of PCD-related processes. No studies have reported previously the isolation and transfection of nucellus protoplasts. In this study, conditions for the isolation and transfection of maize nucellus protoplasts were established. The maize protoplast system can be used for protein expression, localization, and protein-protein interaction. We applied this system to investigate PCD-related processes. Quantitative real-time PCR analysis revealed that transient expression of MADS29 in the maize nucellus protoplast increases Cys-protease gene transcript level. In addition, β-glucuronidase and luciferase activity assays showed that MADS29 could enhance the promoter activities of the Cys-protease gene. Thus, we demonstrated the potential of a highly efficient maize nucellus protoplast system for transient gene expression and investigation of PCD-related processes.
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