ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2015
Structural analysis of alterations in zebrafish muscle differentiation induced by simvastatin and their recovery with cholesterol.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- A transcriptional and regulatory map of mouse somite maturation.Developmental cell · 2023Article
- EmbryoNet: using deep learning to link embryonic phenotypes to signaling pathways.Nature methods · 2023Article
- Elucidating the Effects of the Lipids Regulators Fibrates and Statins on the Health Status of Finfish Species: A Review.Animals : an open access journal from MDPI · 2023Review
- Article
- Assessment of the Preventive Effect of L-carnitine on Post-statin Muscle Damage in a Zebrafish Model.Cells · 2022Article
- Simvastatin and Muscle: Zebrafish and Chicken Show that the Benefits are not Worth the Damage.Frontiers in cell and developmental biology · 2022Article
- Zebrafish as a Model for the Study of Lipid-Lowering Drug-Induced Myopathies.International journal of molecular sciences · 2021Review
- REP1 deficiency causes systemic dysfunction of lipid metabolism and oxidative stress in choroideremia.JCI insight · 2021Article
- Article
- An in vivo brain-bacteria interface: the developing brain as a key regulator of innate immunity.NPJ Regenerative medicine · 2020Article
- "Muscling" Throughout Life: Integrating Studies of Muscle Development, Homeostasis, and Disease in Zebrafish.Current topics in developmental biology · 2017Review
- Alterations in zebrafish development induced by simvastatin: Comprehensive morphological and physiological study, focusing on muscle.Experimental biology and medicine (Maywood, N.J.) · 2016Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro studies show that cholesterol is essential to myogenesis. We have been using zebrafish to overcome the limitations of the in vitro approach and to study the sub-cellular structures and processes involved during myogenesis. We use simvastatin--a drug widely used to prevent high levels of cholesterol and cardiovascular disease--during zebrafish skeletal muscle formation. Simvastatin is an efficient inhibitor of cholesterol synthesis that has various myotoxic consequences. Here, we employed simvastatin concentrations that cause either mild or severe morphological disturbances to observe changes in the cytoskeleton (intermediate filaments and microfilaments), extracellular matrix and adhesion markers by confocal microscopy. With low-dose simvastatin treatment, laminin was almost normal, and alpha-actinin was reduced in the myofibrils. With high simvastatin doses, laminin and vinculin were reduced and appeared discontinuous along the septa, with almost no myofibrils, and small amounts of desmin accumulating close to the septa. We also analyzed sub-cellular alterations in the embryos by electron microscopy, and demonstrate changes in embryo and somite size, septa shape, and in myofibril structure. These effects could be reversed by the addition of exogenous cholesterol. These results contribute to the understanding of the mechanisms of action of simvastatin in muscle cells in particular, and in the study of myogenesis in general.
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