ArticlePloS one2015
Identification and validation of reference genes for quantification of target gene expression with quantitative real-time PCR for tall fescue under four abiotic stresses.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Screening of Reference Gene for RT-qPCR inInternational journal of molecular sciences · 2026Article
- Biostimulant Effects of Rich Mannuronate-Alginate and Their Thermic-Acidic Depolymerized Derivates onPolymers · 2025Article
- Vacuolar phosphatases are essential for efficient nucleotide salvage in Arabidopsis.Journal of experimental botany · 2025Article
- Differential Gene Expression Responses to Salt and Drought Stress in Tall Fescue (Festuca arundinacea Schreb.).Molecular biotechnology · 2024Article
- Selection and Validation of Reference Genes inPlants (Basel, Switzerland) · 2024Article
- Adequate water supply enhances seedling growth and metabolism in Festuca kryloviana: insights from physiological and transcriptomic analys.BMC plant biology · 2024Article
- Comparative proteomics in tall fescue to reveal underlying mechanisms for improving Photosystem II thermotolerance during heat stress memory.BMC genomics · 2024Article
- Selection and validation of reliable reference genes for quantitative real-time PCR in Barnyard millet (Echinochloa spp.) under varied abiotic stress conditions.Scientific reports · 2023Article
- Screening and validating of endogenous reference genes in Chlorella sp. TLD 6B under abiotic stress.Scientific reports · 2023Article
- Evaluation of Reference Genes for Real-Time Quantitative PCR Analysis in Tissues from Bumble Bees (International journal of molecular sciences · 2022Article
- Reference gene selection for quantitative RT-PCR in Miscanthus sacchariflorus under abiotic stress conditions.Molecular biology reports · 2022Article
- Selection and validation of reference genes for quantitative real-time PCR of Quercus mongolica Fisch. ex Ledeb under abiotic stresses.PloS one · 2022Article
- Selection and Evaluation of Candidate Reference Genes for Quantitative Real-Time PCR in Aboveground Tissues and Drought Conditions inFrontiers in genetics · 2022Article
- Expression stability of internal reference gene in response to Trichoderma polysporum infection in Avena fatua L.Current genetics · 2021Article
- Reference genes for quantitative real-time PCR normalization of Cenostigma pyramidale roots under salt stress and mycorrhizal association.Genetics and molecular biology · 2021Article
- Identification and validation of superior housekeeping gene(s) for qRT-PCR data normalization inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Article
- Identifying and validating housekeeping hybrid Prunus spp. genes for root gene-expression studies.PloS one · 2020Article
- Identification and validation of reference genes for real-time quantitative RT-PCR analysis in jute.BMC molecular biology · 2019Article
- Reference genes selection for Calotropis procera under different salt stress conditions.PloS one · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tall fescue (Festuca arundinacea Schreb.) is widely utilized as a major forage and turfgrass species in the temperate regions of the world and is a valuable plant material for studying molecular mechanisms of grass stress tolerance due to its superior drought and heat tolerance among cool-season species. Selection of suitable reference genes for quantification of target gene expression is important for the discovery of molecular mechanisms underlying improved growth traits and stress tolerance. The stability of nine potential reference genes (ACT, TUB, EF1a, GAPDH, SAND, CACS, F-box, PEPKR1 and TIP41) was evaluated using four programs, GeNorm, NormFinder, BestKeeper, and RefFinder. The combinations of SAND and TUB or TIP41 and TUB were most stably expressed in salt-treated roots or leaves. The combinations of GAPDH with TIP41 or TUB were stable in roots and leaves under drought stress. TIP41 and PEPKR1 exhibited stable expression in cold-treated roots, and the combination of F-box, TIP41 and TUB was also stable in cold-treated leaves. CACS and TUB were the two most stable reference genes in heat-stressed roots. TIP41 combined with TUB and ACT was stably expressed in heat-stressed leaves. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) assays of the target gene FaWRKY1 using the identified most stable reference genes confirmed the reliability of selected reference genes. The selection of suitable reference genes in tall fescue will allow for more accurate identification of stress-tolerance genes and molecular mechanisms conferring stress tolerance in this stress-tolerant species.
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