ArticlePloS one2015
Establishment of hairy root cultures by Agrobacterium rhizogenes mediated transformation of Isatis tinctoria L. For the efficient production of flavonoids and evaluation of antioxidant activities.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Gold nanoparticle-mediated elicitation enhances andrographolide biosynthesis via auxin signalling in optimized hairy root cultures of Andrographis paniculata.Protoplasma · 2026Article
- Enhancement of indigotin and indirubin production in root cultures of Isatis species by HScientific reports · 2025Article
- Article
- Research progress on natural plant metabolites targeting apoptosis for endometriosis prevention and treatment: a systematic review.Frontiers in pharmacology · 2025Review
- In vitro strategy to enhance the production of bioactive polyphenols and caffeoylputrescine in the hairy roots of Physalis peruviana L.Scientific reports · 2024Article
- Improvement in the Biosynthesis of Antioxidant-Active Metabolites in In Vitro Cultures ofAntioxidants (Basel, Switzerland) · 2023Article
- Accumulation of phenolic compounds and expression of phenylpropanoid biosynthesis-related genes in leaves of basil transformed withPhysiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins.Plants (Basel, Switzerland) · 2022Review
- Highly efficientFrontiers in plant science · 2022Article
- Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum.Frontiers in plant science · 2022Article
- Phytocannabinoids Biosynthesis in Angiosperms, Fungi, and Liverworts and Their Versatile Role.Plants (Basel, Switzerland) · 2021Review
- Hairy root culture technology: applications, constraints and prospect.Applied microbiology and biotechnology · 2021Review
- Review
- Chitosan elicitation ofIndustrial crops and products · 2018Article
- Ultraviolet radiation for flavonoid augmentation inIndustrial crops and products · 2018Article
- Remarkable enhancement of flavonoid production in a co-cultivation system ofIndustrial crops and products · 2018Article
- Induction of hairy roots byScientia horticulturae · 2016Article
- Cannabis sativa: The Plant of the Thousand and One Molecules.Frontiers in plant science · 2016Review
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7 authors.
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Abstract
In this work, Isatis tinctoria hairy root cultures (ITHRCs) were established as an alternative source for flavonoids (FL) production. I. tinctoria hairy root line V was found to be the most efficient line and was further confirmed by the PCR amplification of rolB, rolC and aux1 genes. Culture parameters of ITHRCs were optimized by Box-Behnken design (BBD), and eight bioactive FL constituents (rutin, neohesperidin, buddleoside, liquiritigenin, quercetin, isorhamnetin, kaempferol and isoliquiritigenin) were quali-quantitatively determined by LC-MS/MS. Under optimal conditions, the total FL accumulation of ITHRCs (24 day-old) achieved was 438.10 μg/g dry weight (DW), which exhibited significant superiority as against that of 2 year-old field grown roots (341.73 μg/g DW). Additionally, in vitro antioxidant assays demonstrated that ITHRCs extracts exhibited better antioxidant activities with lower IC₅₀ values (0.41 and 0.39, mg/mL) as compared to those of field grown roots (0.56 and 0.48, mg/mL). To the best of our knowledge, this is the first report describing FL production and antioxidant activities from ITHRCs.
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