ArticleBioMed research international2015
Hypergravity stimulation enhances PC12 neuron-like cell differentiation.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Modulation of vestibular function and receptor expression by experimental hypergravity in a rat model.Scientific reports · 2025Article
- Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review.International journal of molecular sciences · 2024Review
- Hypergravity enhances RBM4 expression in human bone marrow-derived mesenchymal stem cells and accelerates their differentiation into neurons.Regenerative therapy · 2023Article
- Human neural network activity reacts to gravity changesFrontiers in neuroscience · 2023Article
- The importance of gravity vector on adult mammalian organisms: Effects of hypergravity on mouse testis.PloS one · 2023Article
- Differential Single Cell Responses of Embryonic Stem Cells Versus Embryoid Bodies to Gravity Mechanostimulation.Stem cells and development · 2022Article
- Adaptation and Changes in Actin Dynamics and Cell Motility as Early Responses of Cultured Mammalian Cells to Altered Gravitational Vector.International journal of molecular sciences · 2022Article
- Influence of 40 Hz and 100 Hz Vibration on SH-SY5Y Cells Growth and Differentiation-A Preliminary Study.Molecules (Basel, Switzerland) · 2022Article
- Inhibition of LncRNA Vof-16 expression promotes nerve regeneration and functional recovery after spinal cord injury.Neural regeneration research · 2022Article
- Hypergravity Load Modulates Acetaminophen Nephrotoxicity via Endoplasmic Reticulum Stress in Association with Hepatic microRNA-122 Expression.International journal of molecular sciences · 2021Article
- Physiologically based microenvironment forWorld journal of stem cells · 2018Review
- Enhanced nasal drug delivery efficiency by increasing mechanical loading using hypergravity.Scientific reports · 2018Article
- Hypergravity enhances the therapeutic effect of dexamethasone in allergic asthma and rhinitis animal model.PloS one · 2018Article
- Effects of microgravity on osteoblast mitochondria: a proteomic and metabolomics profile.Scientific reports · 2017Article
- Cold water swimming pretreatment reduces cognitive deficits in a rat model of traumatic brain injury.Neural regeneration research · 2017Article
- Effects of hypergravity on the angiogenic potential of endothelial cells.Journal of the Royal Society, Interface · 2016Article
- Hormetic Effect of Chronic Hypergravity in a Mouse Model of Allergic Asthma and Rhinitis.Scientific reports · 2016Article
- A load of mice to hypergravity causes AMPKα repression with liver injury, which is overcome by preconditioning loads via Nrf2.Scientific reports · 2015Article
- Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system.Neural regeneration research · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Altered gravity is a strong physical cue able to elicit different cellular responses, representing a largely uninvestigated opportunity for tissue engineering/regenerative medicine applications. Our recent studies have shown that both proliferation and differentiation of C2C12 skeletal muscle cells can be enhanced by hypergravity treatment; given these results, PC12 neuron-like cells were chosen to test the hypothesis that hypergravity stimulation might also affect the behavior of neuronal cells, in particular promoting an enhanced differentiated phenotype. PC12 cells were thus cultured under differentiating conditions for either 12 h or 72 h before being stimulated with different values of hypergravity (50 g and 150 g). Effects of hypergravity were evaluated at transcriptional level 1 h and 48 h after the stimulation, and at protein level 48 h from hypergravity exposure, to assess its influence on neurite development over increasing differentiation times. PC12 differentiation resulted strongly affected by the hypergravity treatments; in particular, neurite length was significantly enhanced after exposure to high acceleration values. The achieved results suggest that hypergravity might induce a faster and higher neuronal differentiation and encourage further investigations on the potential of hypergravity in the preparation of cellular constructs for regenerative medicine and tissue engineering purposes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.