ArticleInternational journal of clinical and experimental medicine2015
Effects of α-enolase (ENO1) over-expression on malignant biological behaviors of AGS cells.
Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Impact of Tissue Enolase 1 Protein Overexpression in Esophageal Cancer Progression.International journal of medical sciences · 2021Article
- α-Enolase Lies Downstream of mTOR/HIF1α and Promotes Thyroid Carcinoma Progression by Regulating CST1.Frontiers in cell and developmental biology · 2021Article
- Enolase 1 Correlated With Cancer Progression and Immune-Infiltrating in Multiple Cancer Types: A Pan-Cancer Analysis.Frontiers in oncology · 2020Article
- Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC-803.Oncology letters · 2019Article
- Article
- Proteomic analysis for identifying the differences in molecular profiling between fanconi anaemia and aplastic anaemia.American journal of translational research · 2019Article
- Metabolic reprogramming results in abnormal glycolysis in gastric cancer: a review.OncoTargets and therapy · 2019Review
- ENO1 Acts as a Prognostic Biomarker Candidate and Promotes Tumor Growth and Migration Ability Through the Regulation of Rab1A in Colorectal Cancer.Cancer management and research · 2019Article
- Article
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Authors and funding
8 authors.
Funding
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Abstract
objectiveTo investigate the effects of α-Enolase (ENO1) over-expression on the proliferative and migratory abilities of AGS cells.
methodsThe target gene was cloned and mounted to the eukaryotic expression vector pcDNA3.1(+), then was transfected into gastric cancer cell lines AGS. mRNA and protein level of ENO1 in AGS cells were verified by real-time quantitative RT-PCR and Western Blot, respectively. The effects of over-expression of ENO1 on proliferative and migratory abilities of AGS cells were detected by the experiments of CCK-8, colony formation and wound healing assays.
resultsThe eukaryotic expression vector pcDNA3.1(+)/eno1 was successfully constructed, and verified by sequencing. It was shown from the cell proliferation curves that the proliferative ability of AGS-ENO1 transfected group was higher than that of the control group after 72 hours (t = 3.44, P = 0.04), meanwhile, the number of the cell-colonies of the AGS-ENO1 group were significantly greater than that of the control group (t = 5.26, P = 0.01). For the ability of migration, it was significantly enhanced in the over-expression ENO1 cells than in the negative cells (t = 7.35, P < 0.001).
conclusionThe over-expression of ENO1 protein can enhance the abilities of proliferation and migration in gastric cancer cells of AGS, which indicates that ENO1 may be an important potential tumor-marker associated with the development of gastric cancer.
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Identifiers
25784992PMC4358447What OpenQuestion holds
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