Evidence map›Paper›PMID 25783474›Full record

ArticleThe Journal of general virology2015

Activation and degradation of open reading frame 45 by the replication and transcription activator of Kaposi's sarcoma-associated herpesvirus.

Ying Wang, Kai Yu, Xiuzhi Pei, Tianzheng Zhang, Yuying Guo, Charles Wood, Jinzhong Wang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of general virology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Ying Wang1TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, PR China 2Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, 23 Hongda Street, TEDA, Tianjin 300457, PR China 3Tianjin Key Laboratory of Microbial Functional Genomics, 23 Hongda Street, TEDA, Tianjin 300457, PR China.
Kai Yu1TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, PR China.
Xiuzhi Pei1TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, PR China.
Tianzheng Zhang1TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, PR China.
Yuying Guo1TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, PR China.
Charles Wood4Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln, NE 68583, USA.
Jinzhong Wang1TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, PR China 2Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, 23 Hongda Street, TEDA, Tianjin 300457, PR China 3Tianjin Key Laboratory of Microbial Functional Genomics, 23 Hongda Street, TEDA, Tianjin 300457, PR China.
Nankai University · CNUniversity of Nebraska–Lincoln · US

Funding

KAPOSIS SARCOMA AND HUMAN HERPESVIRUS IN AFRICAR01CA075903 · NCI · UNIVERSITY OF NEBRASKA LINCOLN · PI WOOD, CHARLES · 1998 to 2015
$11.1M
Supplement: Fogarty Training in HIV- and AIDS Associated Diseases/MalignanciesD43TW001429 · FIC · UNIVERSITY OF NEBRASKA LINCOLN · PI WOOD, CHARLES · 2000 to 2015
$7.2M
Nebraska Center for VirologyP30GM103509 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI WOOD, CHARLES · 2012 to 2014
$3.3M
Interferon and Kaposi's sarcoma herpesvirus interactionR01TW007294 · FIC · NANKAI UNIVERSITY · PI WANG, JINZHONG · 2005 to 2009
$260k
FIC NIH HHS D43 TW001429FIC NIH HHS D43TW01492FIC NIH HHS R01 TW007294NCI NIH HHS CA75903NCI NIH HHS R01 CA075903NIGMS NIH HHS GM103509NIGMS NIH HHS P30 GM103509
6 · The paper itself

Abstract

The open reading frame 45 (ORF45) of the Kaposi's sarcoma-associated herpesvirus (KSHV) is an immediate-early phosphorylated tegument protein critical for viral escape from host immune surveillance. Its expression is upregulated by the viral replication and transcription activator (RTA), a key protein that controls the switch from latency to lytic replication. We report here that ORF45 expression was not only upregulated by RTA, but ORF45 could also be degraded by RTA in a proteasome-dependent manner. The ORF45 was activated by RTA via activation of the ORF45 promoter, and the promoter region from nt 69 271 to nt 69 026 was involved. In chronic KSHV infected TRE-BCBL-1 RTA cells, the endogenous ORF45 protein increased dramatically after the induction of RTA expression, but then decreased rapidly after 8 h post-induction. Our study suggests that RTA might control the kinetics of viral replication through fine-tuning of the level of ORF45 and other viral/host proteins.

Indexed as

Gene Expression Regulation, ViralCell LineHerpesvirus 8, HumanHumansImmediate-Early ProteinsProteasome Endopeptidase ComplexProteolysisTrans-ActivatorsTranscription, GeneticImmediate-Early ProteinsORF45 protein, Kaposi's sarcoma-associated herpesvirusProteasome Endopeptidase ComplexRta protein, Human herpesvirus 8Trans-Activators

Identifiers

PMID25783474
PMCPMC4635456
OpenAlexW2065051185

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.