ArticleProtoplasma2016
Establishment of Salvia castanea Diels f. tomentosa Stib. hairy root cultures and the promotion of tanshinone accumulation and gene expression with Ag⁺, methyl jasmonate, and yeast extract elicitation.
Article in Protoplasma, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 99 citations in OpenAlex.
- Phytochemical Analysis and Neuroprotective Effect ofPharmaceuticals (Basel, Switzerland) · 2025Article
- Enhancement of Rosmarinic Acid Production in Hairy Root Cultures ofInternational journal of molecular sciences · 2025Article
- Adventitious and Hairy Root Cultures ofInternational journal of molecular sciences · 2025Article
- Effect of AgMolecules (Basel, Switzerland) · 2024Article
- Accumulation of Polyphenols and Associated Gene Expression in Hairy Roots ofInternational journal of molecular sciences · 2024Article
- Improving Flavonoid Accumulation of Bioreactor-Cultured Adventitious Roots inPlants (Basel, Switzerland) · 2023Article
- Improvement in the Biosynthesis of Antioxidant-Active Metabolites in In Vitro Cultures ofAntioxidants (Basel, Switzerland) · 2023Article
- Article
- Elicitation enhances the production of friedelin and epifriedelanol in hairy root cultures ofFrontiers in plant science · 2023Article
- Isolation ofGenes · 2022Article
- Effect of explant type (leaf, stem) and 2,4-D concentration on callus induction: influence of elicitor type (biotic, abiotic), elicitor concentration and elicitation time on biomass growth rate and costunolide biosynthesis in gazania (Gazania rigens) cell suspension cultures.Bioresources and bioprocessing · 2022Article
- Biotic Elicitors in Adventitious and Hairy Root Cultures: A Review from 2010 to 2022.Molecules (Basel, Switzerland) · 2022Review
- Methyl Jasmonate Activates theMolecules (Basel, Switzerland) · 2022Article
- The Antioxidant, Cytotoxic and Antimicrobial Potential of Phenolic Acids-Enriched Extract of Elicited Hairy Roots ofMolecules (Basel, Switzerland) · 2022Article
- Abietane Diterpenoids from the Hairy Roots ofMolecules (Basel, Switzerland) · 2021Article
- Review
- Increased phenolic acid and tanshinone production and transcriptional responses of biosynthetic genes in hairy root cultures of Salvia przewalskii Maxim. treated with methyl jasmonate and salicylic acid.Molecular biology reports · 2020Article
- Transcriptomic analysis reveals the GRAS family genes respond to gibberellin in Salvia miltiorrhiza hairy roots.BMC genomics · 2020Article
- Production of phenolic acids in hairy root cultures of medicinal plantMolecular biology research communications · 2020Article
- Hairy Root Cultures-A Versatile Tool With Multiple Applications.Frontiers in plant science · 2020Review
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8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salvia castanea Diels f. tomentosa Stib. is an endemic medicinal plant distributed in China, and the notably high content of tanshinone IIA in the root is proven effective for the therapy of heart diseases. Hairy root induction of this Salvia species was inoculated with Agrobacterium rhizogenes strain ATCC 15834. Transformed hairy root was cultured in 6,7-V liquid medium for growth kinetics assessment and elicitation. An S curve was present in the hairy root cultures based on the fresh and dry weights with an interval of 3 days. An optimum concentration of the applied elicitors (15 μM Ag(+), 200 μM methyl jasmonate, and 200 mg l(-1) yeast extract elicitor) benefitted both the growth status and tanshinone accumulation in the hairy root cultures. Tanshinone IIA contents were mostly stimulated 1.8-fold and 1.99-fold compared with the control by Ag(+) and methyl jasmonate elicitation, respectively. Yeast extract dramatically enhanced dry mass accumulation, while it promoted cryptotanshinone content of 2.84 ± 0.33 mg g(-1) dry weight at most in the hairy root cultures. Selected elicitors diversely influenced tanshinone accumulation in the time courses of hairy root cultures within 7 days. Furthermore, transcripts of selected genes in the tanshinone biosynthetic pathway were remarkably upregulated with elicitation. Yeast extract elicitor heightened 13.9-fold of isopentenyl diphosphate isomerase expression level at 12 h, while it increased 16.7-fold of geranylgeranyl diphosphate synthase transcript at 24 h compared with that of the control, which was more effective than Ag(+) and methyl jasmonate. This study provided a convenient hairy root culture system of S. castanea Diels f. tomentosa Stib. for tanshinone production for the first time.
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