ArticleCurrent genetics2015
Gene targeting in the oil-producing fungus Mortierella alpina 1S-4 and construction of a strain producing a valuable polyunsaturated fatty acid.
Article in Current genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- DGLA from the MicroalgaNutrients · 2020Article
- Deletion of the KU70 homologue facilitates gene targeting in Lipomyces starkeyi strain NRRL Y-11558.Current genetics · 2019Article
- Arachidonic acid production by the oleaginous fungusJournal of advanced research · 2018Review
- Efficient gene targeting in non-homologous end-joining-deficient Lipomyces starkeyi strains.Current genetics · 2017Article
- Post-translocational adaptation drives evolution through genetic selection and transcriptional shift in Saccharomyces cerevisiae.Current genetics · 2017Article
- Influence of Supplementation of Vegetable Oil Blends on Omega-3 Fatty Acid Production inBioMed research international · 2017Article
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Authors and funding
9 authors.
Funding
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Abstract
To develop an efficient gene-targeting system in Mortierella alpina 1S-4, we identified the ku80 gene encoding the Ku80 protein, which is involved in the nonhomologous end-joining pathway in genomic double-strand break (DSB) repair, and constructed ku80 gene-disrupted strains via single-crossover homologous recombination. The Δku80 strain from M. alpina 1S-4 showed no negative effects on vegetative growth, formation of spores, and fatty acid productivity, and exhibited high sensitivity to methyl methanesulfonate, which causes DSBs. Dihomo-γ-linolenic acid (DGLA)-producing strains were constructed by disruption of the Δ5-desaturase gene, encoding a key enzyme of bioconversion of DGLA to ARA, using the Δku80 strain as a host strain. The significant improvement of gene-targeting efficiency was not observed by disruption of the ku80 gene, but the construction of DGLA-producing strain by disruption of the Δ5-desaturase gene was succeeded using the Δku80 strain as a host strain. This report describes the first study on the identification and disruption of the ku80 gene in zygomycetes and construction of a DGLA-producing transformant using a gene-targeting system in M. alpina 1S-4.
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