ArticlePlant biotechnology journal2015
Engineered chloroplast dsRNA silences cytochrome p450 monooxygenase, V-ATPase and chitin synthase genes in the insect gut and disrupts Helicoverpa zea larval development and pupation.
Article in Plant biotechnology journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 64 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 163 citations in OpenAlex.
- Chitosan-protected dsRNA increases efficacy against Helicoverpa armigera without compromising biosafety for pollinators.Pest management science · 2026Article
- Evaluation of dsRNA produced inFrontiers in insect science · 2026Article
- Assessment of Potential Toxic Effects of RNAi-Based Transgenic Cotton on the Non-Target PredatorBiology · 2025Article
- Review
- Plant genetic transformation: achievements, current status and future prospects.Plant biotechnology journal · 2025Review
- Vacuolar (HGenes · 2025Article
- Plant immunity to insect herbivores: mechanisms, interactions, and innovations for sustainable pest management.Frontiers in plant science · 2025Review
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Targeting vacuolar ATPase in tobacco whitefly,Journal of biosciences · 2025Review
- SilencingInsects · 2024Article
- Eggplant latent viroid is located in the chloroplasts and nuclei of eggplant infected cells.Virology journal · 2024Article
- Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions.Applied biochemistry and biotechnology · 2024Review
- Article
- Exogenous Application of dsRNA in Plant Protection: Efficiency, Safety Concerns and Risk Assessment.International journal of molecular sciences · 2024Review
- Review
- Plastid dsRNA transgenes trigger phased small RNA-based gene silencing of nuclear-encoded genes.The Plant cell · 2023Article
- Article
- Stabilized Double-Stranded RNA Strategy Improves Cotton Resistance to CBW (International journal of molecular sciences · 2022Article
- Engineering the plastid and mitochondrial genomes of flowering plants.Nature plants · 2022Review
- Engineering chloroplasts for insect pest control.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issuedCorrigendum.2017
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
In the past two decades, chloroplast genetic engineering has been advanced to achieve high-level protein accumulation but not for down-regulation of targeted genes. Therefore, in this report, lepidopteran chitin synthase (Chi), cytochrome P450 monooxygenase (P450) and V-ATPase dsRNAs were expressed via the chloroplast genome to study RNA interference (RNAi) of target genes in intended hosts. PCR and Southern blot analysis confirmed homoplasmy and site-specific integration of transgene cassettes into the chloroplast genomes. Northern blots and real-time qRT-PCR confirmed abundant processed and unprocessed dsRNA transcripts (up to 3.45 million copies of P450 dsRNAs/μg total RNA); the abundance of cleaved dsRNA was greater than the endogenous psbA transcript. Feeding of leaves expressing P450, Chi and V-ATPase dsRNA decreased transcription of the targeted gene to almost undetectable levels in the insect midgut, likely after further processing of dsRNA in their gut. Consequently, the net weight of larvae, growth and pupation rates were significantly reduced by chloroplast-derived dsRNAs. Taken together, successful expression of dsRNAs via the chloroplast genome for the first time opens the door to study RNA interference/processing within plastids. Most importantly, dsRNA expressed in chloroplasts can be utilized for gene inactivation to confer desired agronomic traits or for various biomedical applications, including down-regulation of dysfunctional genes in cancer or autoimmune disorders, after oral delivery of dsRNA bioencapsulated within plant cells.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.