Evidence map›Paper›PMID 25782160›Full record

ArticleInternational journal of molecular sciences2015

Isolation of a pluripotent neural stem cell from the embryonic bovine brain.

Yuhua Gao, Xiangchen Li, Dong Zheng, Weijun Guan, Yuehui Ma

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Chiropteran (Animals : an open access journal from MDPI · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yuhua GaoInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. profzhengdong@yeah.net.
Xiangchen LiInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. profzhengdong@yeah.net.
Dong ZhengCollege of Wildlife Resources, Northeast Forestry University, Harbin 150040, China. profzhengdong@yeah.net.
Weijun GuanInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. weijunguan301@gmail.com.
Yuehui MaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. yuehui_ma@hotmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes).

Indexed as

AnimalsAstrocytesCattleCell DifferentiationCells, CulturedCell SeparationEmbryo, MammalianEye ProteinsHomeodomain ProteinsNerve Tissue ProteinsNestinNeural Stem CellsNeuronsOligodendrogliaPaired Box Transcription FactorsPAX6 Transcription FactorEye ProteinsHomeodomain ProteinsNerve Tissue ProteinsNestinPaired Box Transcription FactorsPAX6 Transcription FactorRepressor ProteinsSOXB1 Transcription Factors

Identifiers

PMID25782160
PMCPMC4394516

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.