Evidence map›Paper›PMID 25781976›Full record

ArticlePloS one2015

Direct detection of fungal siderophores on bats with white-nose syndrome via fluorescence microscopy-guided ambient ionization mass spectrometry.

Samantha J Mascuch, Wilna J Moree, Cheng-Chih Hsu, Gregory G Turner, Tina L Cheng, David S Blehert, A Marm Kilpatrick, Winifred F Frick, Michael J Meehan, Pieter C Dorrestein and 1 more

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
15.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 35 citations in OpenAlex.

  1. Article
  2. Journal of fungi (Basel, Switzerland) · 2025
    Article
  3. Characterization of a High-Affinity Copper TransporterJournal of fungi (Basel, Switzerland) · 2024
    Article
  4. Article
  5. Review
  6. ReducingAnalytical chemistry · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

Samantha J MascuchCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093, United States of America.
Wilna J MoreeSkaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, United States of America.
Cheng-Chih HsuDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, United States of America; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, United States of America.
Gregory G TurnerPennsylvania Game Commission, 2001 Elemerton Ave., Harrisburg, PA 17110, United States of America.
Tina L ChengDepartment of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, CA, United States of America.
David S BlehertUnited States Geological Survey, National Wildlife Health Center, 6006 Schroeder Road, Madison, WI 53711, United States of America.
A Marm KilpatrickDepartment of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, CA, United States of America.
Winifred F FrickDepartment of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, CA, United States of America.
Michael J MeehanDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, United States of America; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, United States of America.
Pieter C DorresteinCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093, United States of America; Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, United States of America; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, United States of America.
Lena GerwickCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093, United States of America.
University of California, San Diego · USUniversity of California, Santa Cruz · USScripps Institution of Oceanography · USNational Wildlife Health Center · USPennsylvania Game Commission

Funding

Training Program in Marine BiotechnologyT32GM067550 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GERWICK, WILLIAM HENRY · 2004 to 2019
$2.0M
Real-Time Imaging of metabolic CommunicationR01GM094802 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DORRESTEIN, PIETER C · 2010 to 2013
$1.0M
NIGMS NIH HHS GM094802NIGMS NIH HHS R01 GM094802NIGMS NIH HHS T32 GM067550
6 · The paper itself

Abstract

White-nose syndrome (WNS) caused by the pathogenic fungus Pseudogymnoascus destructans is decimating the populations of several hibernating North American bat species. Little is known about the molecular interplay between pathogen and host in this disease. Fluorescence microscopy ambient ionization mass spectrometry was used to generate metabolic profiles from the wings of both healthy and diseased bats of the genus Myotis. Fungal siderophores, molecules that scavenge iron from the environment, were detected on the wings of bats with WNS, but not on healthy bats. This work is among the first examples in which microbial molecules are directly detected from an infected host and highlights the ability of atmospheric ionization methodologies to provide direct molecular insight into infection.

Indexed as

AnimalsAscomycotaChiropteraMicroscopy, FluorescenceSiderophoresSpectrometry, Mass, Secondary IonSiderophores

Identifiers

PMID25781976
PMCPMC4364562
OpenAlexW2043810674

What OpenQuestion holds

Textmetadata
LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.