ArticlePloS one2015
A highly efficient recombinant laccase from the yeast Yarrowia lipolytica and its application in the hydrolysis of biomass.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 58 citations in OpenAlex.
- Microbial bioindicators for multidimensional assessment of environmental alterations and ecosystem functioning.World journal of microbiology & biotechnology · 2026Review
- Heterologous Expression of Laccase1 fromMycobiology · 2025Article
- Genomic analysis of the marine yeast Rhodotorula sphaerocarpa ETNP2018 reveals adaptation to the open ocean.BMC genomics · 2023Article
- Laccase-mediated delignification and detoxification of lignocellulosic biomass: removing obstacles in energy generation.Environmental science and pollution research international · 2021Review
- Biotin and ZnIndian journal of microbiology · 2021Article
- A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases.Molecules (Basel, Switzerland) · 2021Review
- Genome-based engineering of ligninolytic enzymes in fungi.Microbial cell factories · 2021Review
- Optimisation of the Production and Bleaching Process for a New Laccase from Madurella mycetomatis, Expressed in Pichia pastoris: from Secretion to Yielding Prominent.Molecular biotechnology · 2021Article
- Heterologous Expression of Laccase FromFrontiers in microbiology · 2020Article
- Genome Sequencing Revealed the Biotechnological Potential of an Obligate ThermophileIndian journal of microbiology · 2019Article
- Characterization of recombinant laccase from Trametes versicolor synthesized by Arxula adeninivorans and its application in the degradation of pharmaceuticals.AMB Express · 2019Article
- Article
- Recovery of laccase-producing gammaproteobacteria from wastewater.Biotechnology reports (Amsterdam, Netherlands) · 2019Article
- Expression of a new laccase from Moniliophthora roreri at high levels in Pichia pastoris and its potential application in micropollutant degradation.AMB Express · 2017Article
- Bio-Prospecting Laccases in the Bacterial Diversity of Activated Sludge From Pulp and Paper Industry.Indian journal of microbiology · 2017Article
- Diverse Metabolic Capacities of Fungi for Bioremediation.Indian journal of microbiology · 2016Review
- Yeast Hosts for the Production of Recombinant Laccases: A Review.Molecular biotechnology · 2016Review
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A modified thermal asymmetric interlaced polymerase chain reaction was performed to obtain the first yeast laccase gene (YlLac) from the isolated yeast Yarrowia lipolytica. The 1557-bp full-length cDNA of YlLac encoded a mature laccase protein containing 519 amino acids preceded by a signal peptide of 19 amino acids, and the YlLac gene was expressed in the yeast Pichia pastoris. YlLac is a monomeric glycoprotein with a molecular mass of ~55 kDa as determined by polyacrylamide-gel electrophoresis. It showed a higher catalytic efficiency towards 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (kcat/Km = 17.5 s(-1) μM(-1)) and 2,6-dimethoxyphenol (kcat/Km = 16.1 s(-1) μM(-1)) than other reported laccases. The standard redox potential of the T1 site of the enzyme was found to be 772 mV. The highest catalytic efficiency of the yeast recombinant laccase, YlLac, makes it a good candidate for industrial applications: it removes phenolic compounds in acid-pretreated woody biomass (Populus balsamifera) and enhanced saccharification.
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