ArticlePloS one2015
The porcine microRNA transcriptome response to transmissible gastroenteritis virus infection.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 18 citations in OpenAlex.
- Comprehensive transcriptomic and metabolomic analysis of porcine intestinal epithelial cells after PDCoV infection.Frontiers in veterinary science · 2024Article
- Clinically informative microRNAs for SARS-CoV-2 infection.Epigenomics · 2023Review
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- miR-215 Targeting Novel GenesGenes · 2020Article
- Article
- mmannot: How to improve small-RNA annotation?PloS one · 2020Article
- Retrospective detection and phylogenetic analysis of swine acute diarrhoea syndrome coronavirus in pigs in southern China.Transboundary and emerging diseases · 2019Article
- Transcriptome analysis of PK-15 cells in innate immune response to porcine deltacoronavirus infection.PloS one · 2019Article
- Establishment of porcine enterocyte/myofibroblast co-cultures for the growth of porcine rota- and coronaviruses.Scientific reports · 2018Article
- Article
- Article
- Virulent duck enteritis virus infected DEF cells generate a unique pattern of viral microRNAs and a novel set of host microRNAs.BMC veterinary research · 2018Article
- MicroRNA transcriptome analysis of porcine vital organ responses to immunosuppressive porcine cytomegalovirus infection.Virology journal · 2018Article
- Transcriptome-wide analysis of immune-responsive microRNAs against poly (I:C) challenge inOncotarget · 2017Article
- Virulence factors in porcine coronaviruses and vaccine design.Virus research · 2016Review
- MicroRNAs in the Host Response to Viral Infections of Veterinary Importance.Frontiers in veterinary science · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transmissible gastroenteritis virus (TGEV; Coronaviridae family) causes huge economic losses to the swine industry. MicroRNAs (miRNAs) play a regulatory role in viral infection and may be involved in the mammalian immune response. Here, we report a comprehensive analysis of host miRNA expression in TGEV-infected swine testis (ST) cells. Deep sequencing generated 3,704,353 and 2,763,665 reads from uninfected ST cells and infected ST cells, respectively. The reads were aligned to known Sus scrofa pre-miRNAs in miRBase 19, identifying 284 annotated miRNAs. Certain miRNAs were differentially regulated during TGEV infection. 59 unique miRNAs displayed significant differentially expression between the normal and TGEV-infected ST cell samples: 15 miRNAs were significantly up-regulated and 44 were significantly down-regulated. Stem-loop RT-PCR was carried out to determine the expression levels of specific miRNAs in the two samples, and the results were consistent with those of sequencing. Gene ontology enrichment analysis of host target genes demonstrated that the differentially expressed miRNAs are involved in regulatory networks, including cellular process, metabolic process, immune system process. This is the first report of the identification of ST cell miRNAs and the comprehensive analysis of the miRNA regulatory mechanism during TGEV infection, which revealed the miRNA molecular regulatory mechanisms for the viral infection, expression of viral genes and the expression of immune-related genes. The results presented here will aid research on the prevention and treatment of viral diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.