ArticlePloS one2015
The effect of low-level laser irradiation on sperm motility, and integrity of the plasma membrane and acrosome in cryopreserved bovine sperm.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- A Comprehensive Systematic Review of the Effects of Photobiomodulation Therapy in Different Light Wavelength Ranges (Blue, Green, Red, and Near-Infrared) on Sperm Cell Characteristics in Vitro and in Vivo.Reproductive sciences (Thousand Oaks, Calif.) · 2024Pooled it
- Influence of biostimulation and temporary weaning on follicular dynamics and pregnancy rates in Nelore cows (Bos taurus indicus).Tropical animal health and production · 2015Trial
- Combining photobiomodulation and naringenin for treatment of asthenozoospermia: A promising therapeutic approach.Lasers in medical science · 2025Article
- Article
- Effects of Varying Light Durations on Sperm Quality in Rams.Animals : an open access journal from MDPI · 2024Article
- The power of 810 nm near-infrared photobiomodulation therapy for human asthenozoospermia.Scientific reports · 2024Article
- β-Nicotinamide mononucleotide improves chilled ram sperm quality in vitro by reducing oxidative stress damage.Animal bioscience · 2024Article
- Photobiomodulation effects on synovial morphology, iNOS gene, and protein expression in a model of acute inflammation.Acta cirurgica brasileira · 2024Article
- Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies.Animals : an open access journal from MDPI · 2022Review
- Restoring Sperm Quality Post-Cryopreservation Using Mitochondrial-Targeted Compounds.Antioxidants (Basel, Switzerland) · 2022Article
- Sperm kinetics of Egyptian buffalo bulls (Journal of advanced veterinary and animal research · 2022Article
- Preclinical and Clinical Applications of Photobiomodulation Therapy in Sperm Motility: A Narrative Review.Journal of lasers in medical sciences · 2022Review
- Photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla.Scientific reports · 2021Article
- The Effect of Laser Acupuncture on Semen Parameters in Infertile Men With Oligospermia: A Randomized Clinical Trial.Journal of lasers in medical sciences · 2021Article
- Photobiomodulation therapy for male infertility.Lasers in medical science · 2020Review
- Grape Seed Procyanidin Extract (GSPE) Improves Goat Sperm Quality When Preserved at 4 °C.Animals : an open access journal from MDPI · 2019Article
- Does low-level laser therapy on degenerated ovine testes improve post-thawed sperm characteristics?Lasers in medical science · 2019Article
- Sperm motility is enhanced by Low Level Laser and Light Emitting Diode photobiomodulation with a dose-dependent response and differential effects in fresh and frozen samples.Laser therapy · 2018Article
- Effectiveness of low level laser therapy for treating male infertility.BioMedicine · 2018Article
- Red light improves spermatozoa motility and does not induce oxidative DNA damage.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectiveFreezing changes sperm integrity remarkably. Cryopreservation involves cooling, freezing, and thawing and all these contribute to structural damage in sperm, resulting in reduced fertility potential. Low-level laser irradiation (LLLI) could increase energy supply to the cell and cause reactive oxygen species reduction (ROS), contributing to the restoration of oxygen consumption and adenosine triphosphate synthesis (ATP) in the mitochondria. Our goal was to analyze the effects of low-level laser irradiation on sperm motility and integrity of the plasma membrane and acrosome in cryopreserved bovine sperm. STUDY DESIGN/MATERIALS AND
methodsWe analyzed 09 samples of bull semen (Bos taurus indicus), divided into three groups: a control group without laser irradiation, a 4J group subjected to a laser irradiation dose of 4 joules, and a 6J group subjected to dose of 6 joules. Samples were divided for the analysis of cell viability and acrosomal membrane integrity using flow cytometry; another portion was used for motion analysis. Irradiation was performed in petri dishes of 30 mm containing 3 ml of semen by an aluminum gallium indium phosphide laser diode with a wavelength of 660 nm, 30 mW power, and energy of 4 and 6 joules for 80 and 120 seconds respectively. Subsequently, the irradiated and control semen samples were subjected to cryopreservation and analyzed by flow cytometry (7AAD and FITC-PSA) using the ISAS--Integrated Semen Analysis System.
resultsFlow cytometry showed an increase in the percentage of live sperm cells and acrosome integrity in relation to control cells when subjected to irradiation of low-power laser in two different doses of 4 and 6 joules (p < 0.05). In the analysis of straightness, percentage of cell movement, and motility, a dose of 4 joules was more effective (p < 0.05).
conclusionWe conclude that LLLI may exert beneficial effects in the preservation of live sperm. A dose of 4 joules prior to cryopreservation was more effective than a dose of 6 joules in preserving sperm motility.
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