Evidence map›Paper›PMID 25780741›Full record

ArticleBiomedical optics express2015

Three-wavelength light control of freely moving Drosophila Melanogaster for less perturbation and efficient social-behavioral studies.

Yen-Yin Lin, Ming-Chin Wu, Po-Yen Hsiao, Li-An Chu, Mei-Mei Yang, Chien-Chung Fu, Ann-Shyn Chiang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Toward Whole-Body Connectomics.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016
    Review
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yen-Yin LinBrain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; Institute of Photonics Technologies, National Tsing Hua University, Hsinchu 30013, Taiwan ; Department of Electrical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan ; These authors contributed equally to this work.
Ming-Chin WuBrain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; These authors contributed equally to this work.
Po-Yen HsiaoInstitute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan ; These authors contributed equally to this work.
Li-An ChuInstitute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan.
Mei-Mei YangDepartment of Art and Creative Design, Hsuan Chuang University, Hsinchu 30013, Taiwan.
Chien-Chung FuBrain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan ; Institute of Nanotechnology and Microsystems Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan ; ccfu@mx.nthu.edu.tw.
Ann-Shyn ChiangBrain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan ; Genomics Research Center, Academia Sinica, Nankang, Taipei 11529, Taiwan ; Kavli Institute for Brain and Mind, University of California, San Diego, La Jolla, CA 92093-0526, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed a real-time automated laser-tracking system combined with continuous wave 1064-nm infrared or 473-nm blue lasers to provide punishment for studying memory in Drosophila Melanogaster. Combining optogenetic tools with laser properties, such as 473-nm and 593-nm lasers that activate light sensitive proteins in artificial transgenic flies, we can manipulate the specific neuron of an assigned fly among multiple flies to investigate neuron circuit relationships in social interactions. In restraining condition assay or optogenetic experiments, a ventral irradiated system would be more efficient due to higher ventral cuticle transmissions and neuron ganglia locations. Therefore, ventral irradiated systems cause less perturbation during behavior studies.

Indexed as

(100.5010) Pattern recognition(170.1420) Biology(170.2655) Functional monitoring and imaging(170.3660) Light propagation in tissues

Identifiers

PMID25780741
PMCPMC4354601

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.