ArticleBiomedical optics express2015
A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging.
Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 126 citations in OpenAlex.
- Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.Journal of biomedical optics · 2026Article
- Mode-resolved picosecond single-photon polarimetry maps modal dynamics in multimode fibers.Nature communications · 2026Article
- Development of three-colour FRET cascade for force sensing of the putative RIAM-vinculin interaction in fibroblasts.Communications chemistry · 2025Article
- Segmentation-guided photon pooling enables robust single cell analysis and fast fluorescence lifetime imaging microscopy.bioRxiv : the preprint server for biology · 2025Article
- High area and volumetric throughput parallel two-photon fluorescence microscopy using silicon photomultiplier arrays.Photonics research · 2025Article
- Rapid Acquisition of High-Pixel Fluorescence Lifetime Images of Living Cells via Image Reconstruction Based on Edge-Preserving Interpolation.Biosensors · 2025Article
- A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos.Scientific reports · 2024Article
- Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding.Advanced drug delivery reviews · 2023Review
- Light-sheet autofluorescence lifetime imaging with a single-photon avalanche diode array.Journal of biomedical optics · 2023Article
- Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM).Nature communications · 2023Article
- A method for the fast and photon-efficient analysis of time-domain fluorescence lifetime image data over large dynamic ranges.Journal of microscopy · 2022Article
- Computational Proposal for Tracking Multiple Molecules in a Multifocus Confocal Setup.ACS photonics · 2022Article
- NAD(P)H fluorescence lifetime imaging of live intestinal nematodes reveals metabolic crosstalk between parasite and host.Scientific reports · 2022Article
- Live-cell fluorescence spectral imaging as a data science challenge.Biophysical reviews · 2022Review
- FRET Ratiometric Nanoprobes for Nanoparticle Monitoring.Biosensors · 2021Review
- Building a Total Internal Reflection Microscope (TIRF) with Active Stabilization (Feedback SMLM).Bio-protocol · 2021Article
- Biochemical resolving power of fluorescence lifetime imaging: untangling the roles of the instrument response function and photon-statistics.Biomedical optics express · 2021Article
- Instant FLIM enables 4DOptica · 2021Article
- Dynamic fluorescence lifetime sensing with CMOS single-photon avalanche diode arrays and deep learning processors.Biomedical optics express · 2021Article
- Fast Gating for Raman Spectroscopy.Sensors (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 1 country.
Funding
Abstract
We demonstrate diffraction limited multiphoton imaging in a massively parallel, fully addressable time-resolved multi-beam multiphoton microscope capable of producing fluorescence lifetime images with sub-50ps temporal resolution. This imaging platform offers a significant improvement in acquisition speed over single-beam laser scanning FLIM by a factor of 64 without compromising in either the temporal or spatial resolutions of the system. We demonstrate FLIM acquisition at 500 ms with live cells expressing green fluorescent protein. The applicability of the technique to imaging protein-protein interactions in live cells is exemplified by observation of time-dependent FRET between the epidermal growth factor receptor (EGFR) and the adapter protein Grb2 following stimulation with the receptor ligand. Furthermore, ligand-dependent association of HER2-HER3 receptor tyrosine kinases was observed on a similar timescale and involved the internalisation and accumulation or receptor heterodimers within endosomes. These data demonstrate the broad applicability of this novel FLIM technique to the spatio-temporal dynamics of protein-protein interaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.