ArticleCell regeneration (London, England)2015
ETV2 expression increases the efficiency of primitive endothelial cell derivation from human embryonic stem cells.
Article in Cell regeneration (London, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
- Advances in vascularized organoids.Chinese medical journal · 2026Review
- ETS-guided iPSC-endothelial models recapitulate malaria pathogenesis.EMBO molecular medicine · 2026Article
- Search-and-remove genome editing allows selection of cells by DNA sequence.Nature communications · 2025Article
- ETV2 Overexpression Promotes Efficient Differentiation of Pluripotent Stem Cells to Endothelial Cells.Biotechnology and bioengineering · 2025Article
- Identification of Cell Fate Determining Transcription Factors for Generating Brain Endothelial Cells.Stem cell reviews and reports · 2025Article
- A streamlined method to generate endothelial cells from human pluripotent stem cells via transient doxycycline-inducible ETV2 activation.Angiogenesis · 2024Article
- Effect and mechanism of hypoxia on differentiation of porcine-induced pluripotent stem cells into vascular endothelial cells.In vitro cellular & developmental biology. Animal · 2024Article
- ETV2 Upregulation Marks the Specification of Early Cardiomyocytes and Endothelial Cells During Co-differentiation.Stem cells (Dayton, Ohio) · 2023Article
- Human Induced Pluripotent Stem Cell-Derived Vascular Cells: Recent Progress and Future Directions.Journal of cardiovascular development and disease · 2021Review
- Programmatic introduction of parenchymal cell types into blood vessel organoids.Stem cell reports · 2021Article
- Selection of different endothelialization modes and different seed cells for tissue-engineered vascular graft.Bioactive materials · 2021Review
- Co-growth of Stem Cells With Target Tissue Culture as an Easy and Effective Method of Directed Differentiation.Frontiers in bioengineering and biotechnology · 2021Article
- Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA.Science advances · 2020Article
- Strategies for derivation of endothelial lineages from human stem cells.Stem cell research & therapy · 2019Review
- Engineering vascularized skeletal muscle tissue with transcriptional factor ETV2-induced autologous endothelial cells.Protein & cell · 2019Article
- Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout.Cell systems · 2018Article
- Requisite endothelial reactivation and effective siRNA nanoparticle targeting of Etv2/Er71 in tumor angiogenesis.JCI insight · 2018Article
- Etv2 as an essential regulator of mesodermal lineage development.Cardiovascular research · 2017Review
- ETV-2 activated proliferation of endothelial cells and attenuated acute hindlimb ischemia in mice.In vitro cellular & developmental biology. Animal · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundEndothelial cells line the luminal surface of blood vessels and form a barrier between the blood and other tissues of the body. Ets variant 2 (ETV2) is transiently expressed in both zebrafish and mice and is necessary and sufficient for vascular endothelial cell specification. Overexpression of this gene in early zebrafish and mouse embryos results in ectopic appearance of endothelial cells. Ectopic expression of ETV2 in later development results in only a subset of cells responding to the signal.
findingsWe have examined the expression pattern of ETV2 in differentiating human embryonic stem cells (ESCs) to determine when the peak of ETV2 expression occurs. We show that overexpression of ETV2 in differentiating human ESC is able to increase the number of endothelial cells generated when administered during or after the endogenous peak of gene expression.
conclusionsAddition of exogenous ETV2 to human ESCs significantly increased the number of cells expressing angioblast genes without arterial or venous specification. This may be a viable solution to generate in vitro endothelial cells for use in research and in the clinic.
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