ArticlePhysiological reports2015
Disturbed shear stress reduces Klf2 expression in arterial-venous fistulae in vivo.
Article in Physiological reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- The needle-puncture mouse aortocaval AVF model: a state of the art review.Renal failure · 2026Review
- Characterization of Arteriovenous Thrombus Formation and Propagation in a Mouse Arteriovenous Fistula Model.Thrombosis and haemostasis · 2025Article
- Disturbed flow in the juxta-anastomotic area of an arteriovenous fistula correlates with endothelial loss, acute thrombus formation, and neointimal hyperplasia.American journal of physiology. Heart and circulatory physiology · 2024Article
- The rodent models of arteriovenous fistula.Frontiers in cardiovascular medicine · 2024Review
- A central arteriovenous fistula reduces systemic hypertension in a mouse model.JVS-vascular science · 2024Article
- Endothelial Cell TGF-β (Transforming Growth Factor-Beta) Signaling Regulates Venous Adaptive Remodeling to Improve Arteriovenous Fistula Patency.Arteriosclerosis, thrombosis, and vascular biology · 2022Article
- Article
- Arteriovenous Fistula-induced Cardiac Remodeling Shows Cardioprotective Features in Mice.JVS-vascular science · 2021Article
- Venous Mechanical Properties After Arteriovenous Fistulae in Mice.The Journal of surgical research · 2020Article
- A mouse model of stenosis distal to an arteriovenous fistula recapitulates human central venous stenosis.JVS-vascular science · 2020Article
- Distinct subsets of T cells and macrophages impact venous remodeling during arteriovenous fistula maturation.JVS-vascular science · 2020Article
- Murine Model of Central Venous Stenosis using Aortocaval Fistula with an Outflow Stenosis.Journal of visualized experiments : JoVE · 2019Article
- The Mouse Aortocaval Fistula Model with Intraluminal Drug Delivery.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Regulation of Transcription Factors by Reactive Oxygen Species and Nitric Oxide in Vascular Physiology and Pathology.Antioxidants & redox signaling · 2017Review
- Calcification in arteriovenous fistula blood vessels may predict arteriovenous fistula failure: a 5-year follow-up study.International urology and nephrology · 2017Article
- High resolution hemodynamic profiling of murine arteriovenous fistula using magnetic resonance imaging and computational fluid dynamics.Theoretical biology & medical modelling · 2017Article
- Shear-Sensitive Genes in Aortic Valve Endothelium.Antioxidants & redox signaling · 2016Review
Corrections and comments
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Authors and funding
13 authors at 1 institution in 2 countries.
Funding
Abstract
Laminar shear stress (SS) induces an antiproliferative and anti-inflammatory endothelial phenotype and increases Klf2 expression. We altered the diameter of an arteriovenous fistula (AVF) in the mouse model to determine whether increased fistula diameter produces disturbed SS in vivo and if acutely increased disturbed SS results in decreased Klf2 expression. The mouse aortocaval fistula model was performed with 22, 25, or 28 gauge needles to puncture the aorta and the inferior vena cava. Duplex ultrasound was used to examine the AVF and its arterial inflow and venous outflow, and SS was calculated. Arterial samples were examined with western blot, immunohistochemistry, and immunofluorescence analysis for proteins and qPCR for RNA. Mice with larger diameter fistulae had diminished survival but increased AVF patency. Increased SS magnitudes and range of frequencies were directly proportional to the needle diameter in the arterial limb proximal to the fistula but not in the venous limb distal to the fistula, with 22-gauge needles producing the most disturbed SS in vivo. Klf2 mRNA and protein expression was diminished in the artery proximal to the fistula in proportion to increasing SS. Increased fistula diameter produces increased SS magnitude and frequency, consistent with disturbed SS in vivo. Disturbed SS is associated with decreased mRNA and protein expression of Klf2. Disturbed SS and reduced Klf2 expression near the fistula are potential therapeutic targets to improve AVF maturation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.