ArticleGenome research2015
A nucleosome turnover map reveals that the stability of histone H4 Lys20 methylation depends on histone recycling in transcribed chromatin.
Article in Genome research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Molecular mechanism of co-transcriptional H3K36 methylation by SETD2.Nature communications · 2025Article
- Review
- Coordinated neuron-specific splicing events restrict nucleosome engagement of the LSD1 histone demethylase complex.Cell reports · 2025Article
- Histone retention preserves epigenetic marks during heat stress-induced transcriptional memory in plants.The EMBO journal · 2023Article
- Diffusion controls local versus dispersed inheritance of histones during replication and shapes epigenomic architecture.PLoS computational biology · 2023Article
- Different chromatin-scanning modes lead to targeting of compacted chromatin by pioneer factors FOXA1 and SOX2.Cell reports · 2023Article
- The molecular basis of heterochromatin assembly and epigenetic inheritance.Molecular cell · 2023Review
- Histone deacetylation primes self-propagation of heterochromatin domains to promote epigenetic inheritance.Nature structural & molecular biology · 2022Article
- SETD2: from chromatin modifier to multipronged regulator of the genome and beyond.Cellular and molecular life sciences : CMLS · 2022Review
- The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states.Cell reports · 2021Article
- Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes.Nature communications · 2021Article
- Decoding the protein composition of whole nucleosomes with Nuc-MS.Nature methods · 2021Article
- Targeting Epigenetics to Cure HIV-1: Lessons From (and for) Cancer Treatment.Frontiers in cellular and infection microbiology · 2021Review
- The role of FACT in managing chromatin: disruption, assembly, or repair?Nucleic acids research · 2020Review
- Two HIRA-dependent pathways mediate H3.3 de novo deposition and recycling during transcription.Nature structural & molecular biology · 2020Article
- Application of Recombination -Induced Tag Exchange (RITE) to study histone dynamics in human cells.Epigenetics · 2020Article
- H2A.Z is dispensable for both basal and activated transcription in post-mitotic mouse muscles.Nucleic acids research · 2020Article
- Article
- Chromatin maturation of the HIV-1 provirus in primary resting CD4+ T cells.PLoS pathogens · 2020Article
- Epigenome Regulation by Dynamic Nucleosome Unwrapping.Trends in biochemical sciences · 2020Review
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Authors and funding
9 authors.
Funding
Abstract
Nucleosome composition actively contributes to chromatin structure and accessibility. Cells have developed mechanisms to remove or recycle histones, generating a landscape of differentially aged nucleosomes. This study aimed to create a high-resolution, genome-wide map of nucleosome turnover in Schizosaccharomyces pombe. The recombination-induced tag exchange (RITE) method was used to study replication-independent nucleosome turnover through the appearance of new histone H3 and the disappearance or preservation of old histone H3. The genome-wide location of histones was determined by chromatin immunoprecipitation-exonuclease methodology (ChIP-exo). The findings were compared with diverse chromatin marks, including histone variant H2A.Z, post-translational histone modifications, and Pol II binding. Finally, genome-wide mapping of the methylation states of H4K20 was performed to determine the relationship between methylation (mono, di, and tri) of this residue and nucleosome turnover. Our analysis showed that histone recycling resulted in low nucleosome turnover in the coding regions of active genes, stably expressed at intermediate levels. High levels of transcription resulted in the incorporation of new histones primarily at the end of transcribed units. H4K20 was methylated in low-turnover nucleosomes in euchromatic regions, notably in the coding regions of long genes that were expressed at low levels. This transcription-dependent accumulation of histone methylation was dependent on the histone chaperone complex FACT. Our data showed that nucleosome turnover is highly dynamic in the genome and that several mechanisms are at play to either maintain or suppress stability. In particular, we found that FACT-associated transcription conserves histones by recycling them and is required for progressive H4K20 methylation.
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