ArticleScientific reports2015
New insights into the mechanisms of gene electrotransfer--experimental and theoretical analysis.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Regulation of Pore Evolution via Progressive Electroporation Enhanced Intracellular Molecule Transport.Research (Washington, D.C.) · 2026Article
- Effect of degeneration stage on non-viral tissue transfection ofMolecular therapy. Nucleic acids · 2025Article
- Feasibility Study for the Use of Gene Electrotransfer and Cell Electrofusion as a Single-Step Technique for the Generation of Activated Cancer Cell Vaccines.The Journal of membrane biology · 2024Article
- Interplay between Electric Field Strength and Number of Short-Duration Pulses for Efficient Gene Electrotransfer.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Importance of the electrophoresis and pulse energy for siRNA-mediated gene silencing by electroporation in differentiated primary human myotubes.Biomedical engineering online · 2024Article
- Article
- Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis.Pharmaceutics · 2022Review
- Aluminum particles generated during millisecond electric pulse application enhance adenovirus-mediated gene transfer in L929 cells.Scientific reports · 2021Article
- The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model.Biomedical engineering online · 2021Article
- A Novel Method for Controlled Gene Expression via Combined Bleomycin and Plasmid DNA Electrotransfer.International journal of molecular sciences · 2019Article
- Numerical study of gene electrotransfer efficiency based on electroporation volume and electrophoretic movement of plasmid DNA.Biomedical engineering online · 2018Article
- Tumor cell death after electrotransfer of plasmid DNA is associated with cytosolic DNA sensor upregulation.Oncotarget · 2018Article
- Synergistic combinations of short high-voltage pulses and long low-voltage pulses enhance irreversible electroporation efficacy.Scientific reports · 2017Article
- Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples.Scientific reports · 2017Article
- Modelling FUS Mislocalisation in an In Vitro Model of Innervated Human Muscle.Journal of molecular neuroscience : MN · 2017Article
- Involvement of a Rac1-Dependent Macropinocytosis Pathway in Plasmid DNA Delivery by Electrotransfection.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Irreversible electroporation ablation area enhanced by synergistic high- and low-voltage pulses.PloS one · 2017Article
- Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field.Biochemistry and biophysics reports · 2016Article
- Gene Electrotransfer: A Mechanistic Perspective.Current gene therapy · 2016Review
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene electrotransfer is a promising non-viral method of gene delivery. In our in vitro study we addressed open questions about this multistep process: how electropermeabilization is related to electrotransfer efficiency; the role of DNA electrophoresis for contact and transfer across the membrane; visualization and theoretical analysis of DNA-membrane interaction and its relation to final transfection efficiency; and the differences between plated and suspended cells. Combinations of high-voltage and low-voltage pulses were used. We obtained that electrophoresis is required for the insertion of DNA into the permeabilized membrane. The inserted DNA is slowly transferred into the cytosol, and nuclear entry is a limiting factor for optimal transfection. The quantification and theoretical analysis of the crucial parameters reveals that DNA-membrane interaction (NDNA) increases with higher DNA concentration or with the addition of electrophoretic LV pulses while transfection efficiency reaches saturation. We explain the differences between the transfection of cell suspensions and plated cells due to the more homogeneous size, shape and movement of suspended cells. Our results suggest that DNA is either translocated through the stable electropores or enters by electo-stimulated endocytosis, possibly dependent on pulse parameters. Understanding of the mechanisms enables the selection of optimal electric protocols for specific applications.
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