ArticleRNA (New York, N.Y.)2015
Metazoan Maelstrom is an RNA-binding protein that has evolved from an ancient nuclease active in protists.
Article in RNA (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Molecular insights into the Drosophila piRNA pathway via systematic ReLo protein interaction screening and structure prediction.Nucleic acids research · 2025Article
- Article
- Attenuation of Piwil2 induced by hypoxic postconditioning prevents cerebral ischemic injury by inhibiting CREB2 promoter methylation.Brain pathology (Zurich, Switzerland) · 2023Article
- Article
- Maelstrom functions in the production of Siwi-piRISC capable of regulating transposons iniScience · 2022Article
- Optimal RNA binding by Egalitarian, a Dynein cargo adaptor, is critical for maintaining oocyte fate inRNA biology · 2021Article
- Repeated Evolution of Inactive Pseudonucleases in a Fungal Branch of the Dis3/RNase II Family of Nucleases.Molecular biology and evolution · 2021Article
- Multi-omic analysis elucidates the genetic basis of hydrocephalus.Cell reports · 2021Article
- Article
- Maelstrom Represses Canonical Polymerase II Transcription within Bi-directional piRNA Clusters in Drosophila melanogaster.Molecular cell · 2019Article
- piRNA-Guided Genome Defense: From Biogenesis to Silencing.Annual review of genetics · 2018Review
- Giant Reverse Transcriptase-Encoding Transposable Elements at Telomeres.Molecular biology and evolution · 2017Article
- LTR-Retrotransposons from Bdelloid Rotifers Capture Additional ORFs Shared between Highly Diverse Retroelement Types.Viruses · 2017Article
- Multiple LINEs of retrotransposon silencing mechanisms in the mammalian germline.Seminars in cell & developmental biology · 2016Review
- The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease.Nature structural & molecular biology · 2016Article
- One Loop to Rule Them All: The Ping-Pong Cycle and piRNA-Guided Silencing.Trends in biochemical sciences · 2016Review
- MeshAndCollect: an automated multi-crystal data-collection workflow for synchrotron macromolecular crystallography beamlines.Acta crystallographica. Section D, Biological crystallography · 2015Article
- The DNA binding high mobility group box protein family functionally binds RNA.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Piwi-interacting RNAs (piRNAs) guide Piwi argonautes to their transposon targets for silencing. The highly conserved protein Maelstrom is linked to both piRNA biogenesis and effector roles in this pathway. One defining feature of Maelstrom is the predicted MAEL domain of unknown molecular function. Here, we present the first crystal structure of the MAEL domain from Bombyx Maelstrom, which reveals a nuclease fold. The overall architecture resembles that found in Mg(2+)- or Mn(2+)-dependent DEDD nucleases, but a clear distinguishing feature is the presence of a structural Zn(2+) ion coordinated by the conserved ECHC residues. Strikingly, metazoan Maelstrom orthologs across the animal kingdom lack the catalytic DEDD residues, and as we show for Bombyx Maelstrom are inactive as nucleases. However, a MAEL domain-containing protein from amoeba having both sequence motifs (DEDD and ECHC) is robustly active as an exoribonuclease. Finally, we show that the MAEL domain of Bombyx Maelstrom displays a strong affinity for single-stranded RNAs. Our studies suggest that the ancient MAEL nuclease domain evolved to function as an RNA-binding module in metazoan Maelstrom.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.