ArticleScientific reports2015
Quo vadis? Microbial profiling revealed strong effects of cleanroom maintenance and routes of contamination in indoor environments.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 53 citations in OpenAlex.
- Microbial Contamination of Gym Equipment: Diversity Patterns, Temporal Dynamics, Staphylococcus Hotspots, and Device-Level Risk Indices.Pathogens (Basel, Switzerland) · 2026Observational
- Common loss of far-red light photoacclimation in cyanobacteria from hot and cold deserts: a case study in the Chroococcidiopsidales.ISME communications · 2023Article
- Bacterial and fungal bioburden reduction on material surfaces using various sterilization techniques suitable for spacecraft decontamination.Frontiers in microbiology · 2023Article
- Metabolomic and cultivation insights into the tolerance of the spacecraft-associatedFrontiers in microbiology · 2023Article
- Space omics research in Europe: Contributions, geographical distribution and ESA member state funding schemes.iScience · 2022Review
- Phenotypic and genomic assessment of the potential threat of human spaceflight-relevantFrontiers in microbiology · 2022Article
- Qualification of Membrane Filtration for Planetary Protection Flight Implementation.Frontiers in microbiology · 2022Article
- Article
- Microbiome dynamics during the HI-SEAS IV mission, and implications for future crewed missions beyond Earth.Microbiome · 2021Article
- Assessing the Risk of Transfer of Microorganisms at the International Space Station Due to Cargo Delivery by Commercial Resupply Vehicles.Frontiers in microbiology · 2020Article
- Prokaryotic and Fungal Characterization of the Facilities Used to Assemble, Test, and Launch the OSIRIS-REx Spacecraft.Frontiers in microbiology · 2020Article
- The matter of the reproductive microbiome.Obstetric medicine · 2019Review
- Biological Contamination Prevention for Outer Solar System Moons of Astrobiological Interest: What Do We Need to Know?Astrobiology · 2019Article
- Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates.BMC microbiology · 2018Article
- Enriching Beneficial Microbial Diversity of Indoor Plants and Their Surrounding Built Environment With Biostimulants.Frontiers in microbiology · 2018Article
- Minimal Influence of Extracellular DNA on Molecular Surveys of Marine Sedimentary Communities.Frontiers in microbiology · 2018Article
- Microbial biodiversity assessment of the European Space Agency's ExoMars 2016 mission.Microbiome · 2017Article
- Microbial succession in an inflated lunar/Mars analog habitat during a 30-day human occupation.Microbiome · 2016Article
- Microorganisms in Confined Habitats: Microbial Monitoring and Control of Intensive Care Units, Operating Rooms, Cleanrooms and the International Space Station.Frontiers in microbiology · 2016Review
- Functional Metagenomics of Spacecraft Assembly Cleanrooms: Presence of Virulence Factors Associated with Human Pathogens.Frontiers in microbiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Space agencies maintain highly controlled cleanrooms to ensure the demands of planetary protection. To study potential effects of microbiome control, we analyzed microbial communities in two particulate-controlled cleanrooms (ISO 5 and ISO 8) and two vicinal uncontrolled areas (office, changing room) by cultivation and 16S rRNA gene amplicon analysis (cloning, pyrotagsequencing, and PhyloChip G3 analysis). Maintenance procedures affected the microbiome on total abundance and microbial community structure concerning richness, diversity and relative abundance of certain taxa. Cleanroom areas were found to be mainly predominated by potentially human-associated bacteria; archaeal signatures were detected in every area. Results indicate that microorganisms were mainly spread from the changing room (68%) into the cleanrooms, potentially carried along with human activity. The numbers of colony forming units were reduced by up to ~400 fold from the uncontrolled areas towards the ISO 5 cleanroom, accompanied with a reduction of the living portion of microorganisms from 45% (changing area) to 1% of total 16S rRNA gene signatures as revealed via propidium monoazide treatment of the samples. Our results demonstrate the strong effects of cleanroom maintenance on microbial communities in indoor environments and can be used to improve the design and operation of biologically controlled cleanrooms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.