ArticleMolecular reproduction and development2015
PS48 can replace bovine serum albumin in pig embryo culture medium, and improve in vitro embryo development by phosphorylating AKT.
Article in Molecular reproduction and development, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Target Validation Studies of PS48, a PDK-1 Allosteric Agonist, for the Treatment of Alzheimer's Disease Phenotype in APP/PS1 Transgenic Mice.International journal of molecular sciences · 2025Article
- Expression of tripartite motif family-like protein 1 and 2 in early conceptus development and placentation in the pig.Reproduction & fertility · 2025Article
- RNA sequencing and gene co-expression network ofAnimal reproduction · 2024Article
- Disruption of anthrax toxin receptor 1 in pigs leads to a rare disease phenotype and protection from senecavirus A infection.Scientific reports · 2022Article
- Review
- Effects of RAD51-stimulatory compound 1 (RS-1) and its vehicle, DMSO, on pig embryo culture.Reproductive toxicology (Elmsford, N.Y.) · 2021Article
- Transcriptional Profiling of Porcine Blastocysts Produced In Vitro in a Chemically Defined Culture Medium.Animals : an open access journal from MDPI · 2021Article
- Ablation of conceptus PTGS2 expression does not alter early conceptus development and establishment of pregnancy in the pig†.Biology of reproduction · 2020Article
- Applications of omics and nanotechnology to improve pig embryo production in vitro.Molecular reproduction and development · 2019Review
- Contributions of RNA-seq to improveAnimal reproduction · 2019Review
- Glutamine supplementation enhances development of in vitro-produced porcine embryos and increases leucine consumption from the medium.Biology of reproduction · 2018Article
- Pharmacologic treatment of donor cells induced to have a Warburg effect-like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs.Molecular reproduction and development · 2018Article
- Single step production of Cas9 mRNA for zygote injection.BioTechniques · 2018Article
- Inactivation of porcine interleukin-1β results in failure of rapid conceptus elongation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Zygote injection of CRISPR/Cas9 RNA successfully modifies the target gene without delaying blastocyst development or altering the sex ratio in pigs.Transgenic research · 2017Article
- Glycine supplementation in vitro enhances porcine preimplantation embryo cell number and decreases apoptosis but does not lead to live births.Molecular reproduction and development · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The application of embryo-related technology is dependent on in vitro culture systems. Unfortunately, most culture media are suboptimal and result in developmentally compromised embryos. Since embryo development is partially dependent upon Warburg Effect-like metabolism, our goal was to test the response of embryos treated with compounds that are known to stimulate or enhance this Effect. One such compound is 5-(4-chloro-phenyl)-3-phenyl-pent-2-enoic acid (PS48). When added during oocyte maturation, the quality of the resultant embryos was compromised, whereas when added to the culture medium after fertilization, PS48 improved both the percentage of embryos that reach the blastocyst stage and the number of nuclei in those blastocysts. Embryonic PS48 treatment resulted in more phosphorylated v-akt murine thymoma viral oncogene homolog (AKT) in blastocyst-stage embryos as compared to the controls. Further, PS48 could replace bovine serum albumin in embryo culture medium, as demonstrated by high-quality embryos that were developmentally competent. The action of PS48 appears to be via stimulation of phosphoinositide-3 kinase and phosphorylation of AKT, which is consistent with stimulation of the Warburg Effect.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.