ArticleProceedings of the National Academy of Sciences of the United States of America2015
Silencing of end-joining repair for efficient site-specific gene insertion after TALEN/CRISPR mutagenesis in Aedes aegypti.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
83 citing papers in PubMed, 169 citations in OpenAlex.
- A conserved in-frame stop codon acts as a multipotent defense mechanism in alphaviruses.Science advances · 2026Article
- Engineering delivery platforms for CRISPR-Cas and their applications in healthcare, agriculture and beyond.Nanoscale advances · 2026Review
- Monoallelic knockout of r2d2 affects the antiviral RNAi response to Mayaro virus and the reproductive potential in Aedes aegypti.Parasites & vectors · 2026Article
- Leveraging DNA repair mechanisms in genetically engineered biocontrol.Current opinion in insect science · 2026Review
- Plant resistance: scientific basis and latest research progress.Frontiers in plant science · 2026Review
- The Sindbis virus nsP3 opal codon protects viral RNA and fitness by maintaining replication spherule integrity.bioRxiv : the preprint server for biology · 2025Article
- Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide.Frontiers in zoology · 2025Review
- Differential elimination of marked sex chromosomes enables production of nontransgenic male mosquitoes in a single strain.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Manipulation of a New Non-model Insect Genome Using Targeted CRISPR-Era Approaches.Methods in molecular biology (Clifton, N.J.) · 2025Article
- CRISPR-editing of the virus vectorMethodsX · 2024Article
- Identification of a dengue 2 virus envelope protein receptor inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Cytoskeleton-associated gelsolin responds to the midgut distention process in saline meal-fed Aedes aegypti and affects arbovirus dissemination from the midgut.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- Response of the mosquito immune system and symbiotic bacteria to pathogen infection.Parasites & vectors · 2024Review
- A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations.Nature communications · 2024Article
- CRISPR/Cas9: a cutting-edge solution for combatting the fall armyworm, Spodoptera frugiperda.Molecular biology reports · 2023Review
- Aedes aegypti Argonaute 2 controls arbovirus infection and host mortality.Nature communications · 2023Article
- CRISPR-Cas9 Methods and Key Considerations in the Production ofCold Spring Harbor protocols · 2023Article
- RNA interference is essential to modulating the pathogenesis of mosquito-borne viruses in the yellow fever mosquitoProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Optimizing CRE and PhiC31 mediated recombination inFrontiers in bioengineering and biotechnology · 2023Article
- CRISPR-based gene editing of non-homologous end joining factors biases DNA repair pathway choice toward single-strand annealing inCurrent research in biotechnology · 2023Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Conventional control strategies for mosquito-borne pathogens such as malaria and dengue are now being complemented by the development of transgenic mosquito strains reprogrammed to generate beneficial phenotypes such as conditional sterility or pathogen resistance. The widespread success of site-specific nucleases such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 in model organisms also suggests that reprogrammable gene drive systems based on these nucleases may be capable of spreading such beneficial phenotypes in wild mosquito populations. Using the mosquito Aedes aegypti, we determined that mutations in the FokI domain used in TALENs to generate obligate heterodimeric complexes substantially and significantly reduce gene editing rates. We found that CRISPR/Cas9-based editing in the mosquito Ae. aegypti is also highly variable, with the majority of guide RNAs unable to generate detectable editing. By first evaluating candidate guide RNAs using a transient embryo assay, we were able to rapidly identify highly effective guide RNAs; focusing germ line-based experiments only on this cohort resulted in consistently high editing rates of 24-90%. Microinjection of double-stranded RNAs targeting ku70 or lig4, both essential components of the end-joining response, increased recombination-based repair in early embryos as determined by plasmid-based reporters. RNAi-based suppression of Ku70 concurrent with embryonic microinjection of site-specific nucleases yielded consistent gene insertion frequencies of 2-3%, similar to traditional transposon- or ΦC31-based integration methods but without the requirement for an initial docking step. These studies should greatly accelerate investigations into mosquito biology, streamline development of transgenic strains for field releases, and simplify the evaluation of novel Cas9-based gene drive systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.