Evidence map›Paper›PMID 25775562›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2015

New aspect of plant-rhizobia interaction: alkaloid biosynthesis in Crotalaria depends on nodulation.

Simon Irmer, Nora Podzun, Dorothee Langel, Franziska Heidemann, Elisabeth Kaltenegger, Brigitte Schemmerling, Christoph-Martin Geilfus, Christian Zörb, Dietrich Ober

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
5.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 69 citations in OpenAlex.

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  14. CRISPR/Cas9-Mediated Genome Editing in Comfrey (Molecules (Basel, Switzerland) · 2021
    Article
  15. The Orphan CropFrontiers in plant science · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Simon IrmerBiochemical Ecology and Molecular Evolution, Botanical Institute and Botanical Gardens, Kiel University, 24118 Kiel, Germany;
Nora PodzunBiochemical Ecology and Molecular Evolution, Botanical Institute and Botanical Gardens, Kiel University, 24118 Kiel, Germany;
Dorothee LangelBiochemical Ecology and Molecular Evolution, Botanical Institute and Botanical Gardens, Kiel University, 24118 Kiel, Germany;
Franziska HeidemannBiochemical Ecology and Molecular Evolution, Botanical Institute and Botanical Gardens, Kiel University, 24118 Kiel, Germany;
Elisabeth KalteneggerBiochemical Ecology and Molecular Evolution, Botanical Institute and Botanical Gardens, Kiel University, 24118 Kiel, Germany;
Brigitte SchemmerlingBiochemical Ecology and Molecular Evolution, Botanical Institute and Botanical Gardens, Kiel University, 24118 Kiel, Germany;
Christoph-Martin GeilfusInstitute of Plant Nutrition and Soil Science, Kiel University, 24118 Kiel, Germany; and.
Christian ZörbInstitute of Crop Science, Quality of Plant Products, University Hohenheim, 70593 Stuttgart, Germany.
Dietrich OberBiochemical Ecology and Molecular Evolution, Botanical Institute and Botanical Gardens, Kiel University, 24118 Kiel, Germany; dober@bot.uni-kiel.de.
Kiel University · DEUniversity of Hohenheim · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Infection of legume hosts by rhizobial bacteria results in the formation of a specialized organ, the nodule, in which atmospheric nitrogen is reduced to ammonia. Nodulation requires the reprogramming of the plant cell, allowing the microsymbiont to enter the plant tissue in a highly controlled manner. We have found that, in Crotalaria (Fabaceae), this reprogramming is associated with the biosynthesis of pyrrolizidine alkaloids (PAs). These compounds are part of the plant's chemical defense against herbivores and cannot be regarded as being functionally involved in the symbiosis. PAs in Crotalaria are detectable only when the plants form nodules after infection with their rhizobial partner. The identification of a plant-derived sequence encoding homospermidine synthase (HSS), the first pathway-specific enzyme of PA biosynthesis, suggests that the plant and not the microbiont is the producer of PAs. Transcripts of HSS are detectable exclusively in the nodules, the tissue with the highest concentration of PAs, indicating that PA biosynthesis is restricted to the nodules and that the nodules are the source from which the alkaloids are transported to the above ground parts of the plant. The link between nodulation and the biosynthesis of nitrogen-containing alkaloids in Crotalaria highlights a further facet of the effect of symbiosis with rhizobia on the ecologically important trait of the plant's chemical defense.

Indexed as

Plant Root NodulationAlkyl and Aryl TransferasesCrotalariaDNA, ComplementaryNitrogenPlant RootsPolymerase Chain ReactionPyrrolizidine AlkaloidsRecombinant ProteinsRhizobiumSymbiosisAlkyl and Aryl TransferasesDNA, Complementaryhomospermidine synthetaseNitrogenPyrrolizidine AlkaloidsRecombinant Proteinsalkaloid biosynthesischemical defenseplant secondary metabolismpyrrolizidine alkaloidsroot-nodule symbiosis

Identifiers

PMID25775562
PMCPMC4386363
OpenAlexW2063001337

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.