ArticleProceedings of the National Academy of Sciences of the United States of America2015
Genome-wide analysis of thylakoid-bound ribosomes in maize reveals principles of cotranslational targeting to the thylakoid membrane.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 90 citations in OpenAlex.
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- Assembly-dependent translational feedback regulation of photosynthetic proteins in land plants.Nature plants · 2025Article
- The psbA open reading frame acts in cis to toggle HCF173 from an activator to a repressor for light-regulated psbA translation in plants.The Plant cell · 2025Article
- Localization of proteins involved in the biogenesis and repair of the photosynthetic apparatus to thylakoid subdomains inPlant direct · 2024Article
- Translational activation by a synthetic PPR protein elucidates control of psbA translation in Arabidopsis chloroplasts.The Plant cell · 2024Article
- RESISTANCE TO PHYTOPHTHORA1 promotes cytochrome b559 formation during early photosystem II biogenesis in Arabidopsis.The Plant cell · 2024Article
- Chloroplast ATP synthase: From structure to engineering.The Plant cell · 2024Review
- STIC2 selectively binds ribosome-nascent chain complexes in the cotranslational sorting of Arabidopsis thylakoid proteins.The EMBO journal · 2024Article
- Optimization of ribosome profiling in plants including structural analysis of rRNA fragments.Plant methods · 2024Article
- Experimental approaches to studying translation in plant semi-autonomous organelles.Journal of experimental botany · 2024Review
- Chloroplast biogenesis involves spatial coordination of nuclear and organellar gene expression in Chlamydomonas.Plant physiology · 2024Article
- Membrane association of active genes organizes the chloroplast nucleoid structure.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Thylakoid protein FPB1 synergistically cooperates with PAM68 to promote CP47 biogenesis and Photosystem II assembly.Nature communications · 2024Article
- Eukaryote-specific assembly factor DEAP2 mediates an early step of photosystem II assembly in Arabidopsis.Plant physiology · 2023Article
- Plant organellar RNA maturation.The Plant cell · 2023Review
- One-helix protein 2 is not required for the synthesis of photosystem II subunit D1 in Chlamydomonas.Plant physiology · 2023Article
- Chloroplast proteostasis: A story of birth, life, and death.Plant communications · 2023Review
- Characterization of the Evolutionary Pressure onGenes · 2022Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplast genomes encode ∼ 37 proteins that integrate into the thylakoid membrane. The mechanisms that target these proteins to the membrane are largely unexplored. We used ribosome profiling to provide a comprehensive, high-resolution map of ribosome positions on chloroplast mRNAs in separated membrane and soluble fractions in maize seedlings. The results show that translation invariably initiates off the thylakoid membrane and that ribosomes synthesizing a subset of membrane proteins subsequently become attached to the membrane in a nuclease-resistant fashion. The transition from soluble to membrane-attached ribosomes occurs shortly after the first transmembrane segment in the nascent peptide has emerged from the ribosome. Membrane proteins whose translation terminates before emergence of a transmembrane segment are translated in the stroma and targeted to the membrane posttranslationally. These results indicate that the first transmembrane segment generally comprises the signal that links ribosomes to thylakoid membranes for cotranslational integration. The sole exception is cytochrome f, whose cleavable N-terminal cpSecA-dependent signal sequence engages the thylakoid membrane cotranslationally. The distinct behavior of ribosomes synthesizing the inner envelope protein CemA indicates that sorting signals for the thylakoid and envelope membranes are distinguished cotranslationally. In addition, the fractionation behavior of ribosomes in polycistronic transcription units encoding both membrane and soluble proteins adds to the evidence that the removal of upstream ORFs by RNA processing is not typically required for the translation of internal genes in polycistronic chloroplast mRNAs.
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