ArticleProceedings of the National Academy of Sciences of the United States of America2015
Genetic and biochemical investigations of the role of MamP in redox control of iron biomineralization in Magnetospirillum magneticum.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 65 citations in OpenAlex.
- Cross-linking proteomics analysis for reliable identification of MamXY-associated magnetosome formation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Biosynthesis and function of magnetic organelles in magnetotactic bacteria.Nature reviews. Microbiology · 2026Review
- Magnetochrome-catalyzed oxidation of ferrous iron by MamP enables magnetite crystal growth in the magnetotactic bacterium AMB-1.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- An Integrated Approach to Elucidate the Interplay between Iron Uptake Dynamics and Magnetosome Formation at the Single-Cell Level inACS applied materials & interfaces · 2024Article
- Hybrid Hydroxyapatite-Metal Complex Materials Derived from Amino Acids and Nucleobases.Molecules (Basel, Switzerland) · 2024Review
- Understanding Biomineralization Mechanisms to Produce Size-Controlled, Tailored Nanocrystals for Optoelectronic and Catalytic Applications: A Review.ACS applied nano materials · 2024Review
- A protease-mediated switch regulates the growth of magnetosome organelles inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Amperometric Biosensors Based on Direct Electron Transfer Enzymes.Molecules (Basel, Switzerland) · 2021Review
- Identification and Genomic Characterization of Two Previously Unknown MagnetotacticFrontiers in microbiology · 2021Article
- Magnetotactic Bacteria Accumulate a Large Pool of Iron Distinct from Their Magnetite Crystals.Applied and environmental microbiology · 2020Article
- A Compass To Boost Navigation: Cell Biology of Bacterial Magnetotaxis.Journal of bacteriology · 2020Review
- Diversity of Uncultured Magnetospirillum sp. from the Sediments of South Kerala Sedimentary Basin, India.Current microbiology · 2020Article
- Magnetic genes: Studying the genetics of biomineralization in magnetotactic bacteria.PLoS genetics · 2020Review
- Direct Electron Transfer of Enzymes Facilitated by Cytochromes.ChemElectroChem · 2019Review
- Integrated transcriptomic and proteomic analyses of a molecular mechanism of radular teeth biomineralization in Cryptochiton stelleri.Scientific reports · 2019Article
- Reducing Conditions Favor Magnetosome Production inFrontiers in microbiology · 2019Article
- Novel Protein Mg2046 Regulates Magnetosome Synthesis inFrontiers in microbiology · 2019Article
- High-Throughput Microfluidic Sorting of Live Magnetotactic Bacteria.Applied and environmental microbiology · 2018Article
- Bacterial encapsulins as orthogonal compartments for mammalian cell engineering.Nature communications · 2018Article
- Magnetoreception-A sense without a receptor.PLoS biology · 2017Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Magnetotactic bacteria have evolved complex subcellular machinery to construct linear chains of magnetite nanocrystals that allow the host cell to sense direction. Each mixed-valent iron nanoparticle is mineralized from soluble iron within a membrane-encapsulated vesicle termed the magnetosome, which serves as a specialized compartment that regulates the iron, redox, and pH environment of the growing mineral. To dissect the biological components that control this process, we have carried out a genetic and biochemical study of proteins proposed to function in iron mineralization. In this study, we show that the redox sites of c-type cytochromes of the Magnetospirillum magneticum AMB-1 magnetosome island, MamP and MamT, are essential to their physiological function and that ablation of one or both heme motifs leads to loss of function, suggesting that their ability to carry out redox chemistry in vivo is important. We also develop a method to heterologously express fully heme-loaded MamP from AMB-1 for in vitro biochemical studies, which show that its Fe(III)-Fe(II) redox couple is set at an unusual potential (-89 ± 11 mV) compared with other related cytochromes involved in iron reduction or oxidation. Despite its low reduction potential, it remains competent to oxidize Fe(II) to Fe(III) and mineralize iron to produce mixed-valent iron oxides. Finally, in vitro mineralization experiments suggest that Mms mineral-templating peptides from AMB-1 can modulate the iron redox chemistry of MamP.
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