ArticleProceedings of the National Academy of Sciences of the United States of America2015
MiR-2 family regulates insect metamorphosis by controlling the juvenile hormone signaling pathway.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 122 citations in OpenAlex.
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- A time course analysis through diapause reveals dynamic temporal patterns of microRNAs associated with endocrine regulation in the butterfly Pieris napi.Molecular ecology · 2025Article
- Neuropeptide Ecdysis-Triggering Hormone and Its Receptor Mediate the Fecundity Improvement of 'Candidatus Liberibacter Asiaticus'-Infected Diaphorina citri Females and CLas Proliferation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Transcriptomic Characterization of miRNAs inGenes · 2025Article
- miR-34-5p mediates 20E-induced autophagy in the fat body of Bombyx mori by targeting Atg1.BMC genomics · 2025Article
- Interactions of juvenile hormone, 20-hydroxyecdysone, developmental genes, and miRNAs during pupal development in Apis mellifera.Scientific reports · 2025Article
- Developmental Expression Patterns of miRNA inGenes · 2025Article
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- Regulation of soldier caste differentiation by microRNAs in Formosan subterranean termite (PeerJ · 2024Article
- Investigating the role of aae-miR-34-5p in the regulation of juvenile hormone biosynthesis genes in the mosquito Aedes aegypti.Scientific reports · 2023Article
- Identification and Characterization of Development-Related microRNAs in the Red Flour Beetle,International journal of molecular sciences · 2023Article
- The MicroRNA Ame-Bantam-3p Controls Larval Pupal Development by Targeting the Multiple Epidermal Growth Factor-like Domains 8 Gene (International journal of molecular sciences · 2023Article
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- miR-275/305 cluster is essential for maintaining energy metabolic homeostasis by the insulin signaling pathway in Bactrocera dorsalis.PLoS genetics · 2022Article
- miR-309a is a regulator of ovarian development in the oriental fruit fly Bactrocera dorsalis.PLoS genetics · 2022Article
- Identification and profiling of stable microRNAs in hemolymph of young and oldCurrent research in insect science · 2022Article
- Review
- Spatio-Temporal Profiling ofJournal of fungi (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In 2009 we reported that depletion of Dicer-1, the enzyme that catalyzes the final step of miRNA biosynthesis, prevents metamorphosis in Blattella germanica. However, the precise regulatory roles of miRNAs in the process have remained elusive. In the present work, we have observed that Dicer-1 depletion results in an increase of mRNA levels of Krüppel homolog 1 (Kr-h1), a juvenile hormone-dependent transcription factor that represses metamorphosis, and that depletion of Kr-h1 expression in Dicer-1 knockdown individuals rescues metamorphosis. We have also found that the 3'UTR of Kr-h1 mRNA contains a functional binding site for miR-2 family miRNAs (for miR-2, miR-13a, and miR-13b). These data suggest that metamorphosis impairment caused by Dicer-1 and miRNA depletion is due to a deregulation of Kr-h1 expression and that this deregulation is derived from a deficiency of miR-2 miRNAs. We corroborated this by treating the last nymphal instar of B. germanica with an miR-2 inhibitor, which impaired metamorphosis, and by treating Dicer-1-depleted individuals with an miR-2 mimic to allow nymphal-to-adult metamorphosis to proceed. Taken together, the data indicate that miR-2 miRNAs scavenge Kr-h1 transcripts when the transition from nymph to adult should be taking place, thus crucially contributing to the correct culmination of metamorphosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.