ArticlePloS one2015
The use of genus-specific amplicon pyrosequencing to assess phytophthora species diversity using eDNA from soil and water in Northern Spain.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- The potential of methylation signal detection in eDNA toward functional ecological monitoring.Communications biology · 2026Review
- Catching the ElusiveBiotech (Basel (Switzerland)) · 2026Review
- Molecular Identification and RNA-Based Management of Fungal Plant Pathogens: From PCR to CRISPR/Cas9.International journal of molecular sciences · 2026Review
- Oomycete Diversity and Ecology in Declining Alder Stands in Switzerland.Microbial ecology · 2025Article
- Climate acts as an environmental filter to plant pathogens.The ISME journal · 2024Article
- Oomycete Soil Diversity Associated withJournal of fungi (Basel, Switzerland) · 2023Article
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- Extensive morphological and behavioural diversity among fourteen new and seven described species inPersoonia · 2022Article
- Comparison of Primers for the Detection ofJournal of fungi (Basel, Switzerland) · 2022Article
- New Reports ofPathogens (Basel, Switzerland) · 2022Article
- DNA Metabarcoding and Isolation by Baiting Complement Each Other in RevealingJournal of fungi (Basel, Switzerland) · 2022Article
- Application of Real-Time PCR for the Detection and Quantification of Oomycetes in Ornamental Nursery Stock.Journal of fungi (Basel, Switzerland) · 2021Article
- Metagenomics Approaches for the Detection and Surveillance of Emerging and Recurrent Plant Pathogens.Microorganisms · 2021Review
- Phytophthora Species Associated with Roots of Native and Non-native Trees in Natural and Managed Forests.Microbial ecology · 2021Article
- Two new Nothophytophthora species from streams in Ireland and Northern Ireland: Nothophytophthora irlandica and N. lirii sp. nov.PloS one · 2021Article
- Association ofMicroorganisms · 2020Article
- High-throughput identification and diagnostics of pathogens and pests: Overview and practical recommendations.Molecular ecology resources · 2019Review
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- Canker and decline diseases caused by soil- and airbornePersoonia · 2018Review
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3 authors.
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Abstract
Phytophthora is one of the most important and aggressive plant pathogenic genera in agriculture and forestry. Early detection and identification of its pathways of infection and spread are of high importance to minimize the threat they pose to natural ecosystems. eDNA was extracted from soil and water from forests and plantations in the north of Spain. Phytophthora-specific primers were adapted for use in high-throughput Sequencing (HTS). Primers were tested in a control reaction containing eight Phytophthora species and applied to water and soil eDNA samples from northern Spain. Different score coverage threshold values were tested for optimal Phytophthora species separation in a custom-curated database and in the control reaction. Clustering at 99% was the optimal criteria to separate most of the Phytophthora species. Multiple Molecular Operational Taxonomic Units (MOTUs) corresponding to 36 distinct Phytophthora species were amplified in the environmental samples. Pyrosequencing of amplicons from soil samples revealed low Phytophthora diversity (13 species) in comparison with the 35 species detected in water samples. Thirteen of the MOTUs detected in rivers and streams showed no close match to sequences in international sequence databases, revealing that eDNA pyrosequencing is a useful strategy to assess Phytophthora species diversity in natural ecosystems.
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