Evidence map›Paper›PMID 25775014›Full record

ArticlePloS one2015

Identification of protein tyrosine phosphatase receptor gamma extracellular domain (sPTPRG) as a natural soluble protein in plasma.

Elisabetta Moratti, Marzia Vezzalini, Luisa Tomasello, Davide Giavarina, Claudio Sorio

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 24 citations in OpenAlex.

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  4. Extracellular vesicles as mediators and markers of acute organ injury: current concepts.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Elisabetta MorattiDepartment of Pathology and Diagnostics, University of Verona, Verona, Italy.
Marzia VezzaliniDepartment of Pathology and Diagnostics, University of Verona, Verona, Italy.
Luisa TomaselloDepartment of Pathology and Diagnostics, University of Verona, Verona, Italy.
Davide GiavarinaClinical Chemistry and Hematology Laboratory St. Bortolo Hospital, Vicenza, Italy.
Claudio SorioDepartment of Pathology and Diagnostics, University of Verona, Verona, Italy.
University of Verona · ITOspedale San Bortolo · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPTPRG is a widely expressed protein tyrosine phosphatase present in various isoforms. Peptides from its extracellular domain have been detected in plasma by proteomic techniques. We aim at characterizing the plasmatic PTPRG (sPTPRG) form and to identify its source. METHODOLOGY/PRINCIPAL

findingsThe expression of sPTPRG was evaluated in human plasma and murine plasma and tissues by immunoprecipitation and Western blotting. The polypeptides identified have an apparent Mr of about 120 kDa (major band) and 90 kDa (minor band) respectively. Full length PTPRG was identified in the 100.000×g pelleted plasma fraction, suggesting that it was present associated to cell-derived vesicles (exosomes). The release of sPTPRG by HepG2 human hepatocellular carcinoma cell line was induced by ethanol and sensitive to metalloproteinase and not to Furin inhibitors. Finally, increased levels of the plasmatic ∼120 kDa isoform were associated with the occurrence of liver damage.

conclusionsThese results demonstrate that sPTPRG represent a novel candidate protein biomarker in plasma whose increased expression is associated to hepatocyte damage. This observation could open a new avenue of investigation in this challenging field.

Indexed as

AnimalsBiomarkersEthanolExosomesHepatocytesHep G2 CellsHumansMaleMiceProtein IsoformsProteomeReceptor-Like Protein Tyrosine Phosphatases, Class 5BiomarkersEthanolProtein IsoformsProteomePTPRG protein, humanPtprg protein, mouseReceptor-Like Protein Tyrosine Phosphatases, Class 5

Identifiers

PMID25775014
PMCPMC4361625
OpenAlexW2047276059

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.