ArticlePloS one2015
Osteocytic protein expression response to doxercalciferol therapy in pediatric dialysis patients.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Hyperphosphatemia and Chronic Kidney Disease: A Major Daily Concern Both in Adults and in Children.Calcified tissue international · 2021Review
- Article
- Treatment of hyperphosphatemia: the dangers of high PTH levels.Pediatric nephrology (Berlin, Germany) · 2020Review
- The Importance of Biologically Active Vitamin D for Mineralization by Osteocytes After Parathyroidectomy for Renal Hyperparathyroidism.JBMR plus · 2019Article
- Article
- Bone and heart health in chronic kidney disease: role of dentin matrix protein 1.Current opinion in nephrology and hypertension · 2019Review
- CKD-MBD: from the Pathogenesis to the Identification and Development of Potential Novel Therapeutic Targets.Current osteoporosis reports · 2018Review
- Impaired osteocyte maturation in the pathogenesis of renal osteodystrophy.Kidney international · 2018Article
- Fibroblast Growth Factor 23 Regulation by Systemic and Local Osteoblast-Synthesized 1,25-Dihydroxyvitamin D.Journal of the American Society of Nephrology : JASN · 2017Article
- Interactions of sclerostin with FGF23, soluble klotho and vitamin D in renal transplantation.PloS one · 2017Article
- Altered Osteocyte-Specific Protein Expression in Bone after Childhood Solid Organ Transplantation.PloS one · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundOsteocytic protein expression is dysregulated in CKD and is affected by changes in mineral metabolism; however the effects of active vitamin D sterol therapy on osteocyte protein expression in advanced CKD is unknown.
methodsEleven pediatric patients with end stage kidney disease underwent bone biopsy, were treated for 8 months with doxercalciferol, and then underwent a second bone biopsy. Bone expression of fibroblast growth factor 23 (FGF23), dentin matrix protein 1 (DMP1), and sclerostin were determined by immunohistochemistry and quantified by Ariol Scanning. Western blot analysis and qRT-PCR was performed on bone abstracts of a subset of study subjects to determine the nature (i.e. size) of FGF23 and DMP1 in bone before and after therapy.
resultsAs assessed by immunohistochemistry, bone FGF23, DMP1 and sclerostin protein all increased with therapy. In the case of FGF23, this increase was due to an increase in the full-length molecule without the appearance of FGF23 fragments. DMP1 was present primarily in its full-length form in healthy controls while 57kDa and 37kDa fragments of DMP1 were apparent in bone of dialysis patients at baseline and the 57 kDa appeared to decrease with therapy.
conclusionMarked changes in osteocytic protein expression accompany doxercalciferol therapy, potentially impacting bone mineralization and the skeletal response to PTH. The effects of these bone changes on long-term outcomes remain to be determined.
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