ArticlePloS one2015
Genome editing using TALENs in blind Mexican Cavefish, Astyanax mexicanus.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 72 citations in OpenAlex.
- Analysis of multi-trait evolution across independently evolved cavefish populations reveals shared and independent evolution of suites of traits.Proceedings. Biological sciences · 2026Article
- Analysis of multi-trait evolution across independently evolved cavefish populations reveals shared and independent evolution of suites of cave-associated traits.bioRxiv : the preprint server for biology · 2025Article
- The rx3 Gene Contributes to the Evolution of Eye Loss in the Cavefish Astyanax mexicanus.Evolution & development · 2025Article
- Postprandial Sleep in Short-Sleeping Mexican Cavefish.Journal of experimental zoology. Part A, Ecological and integrative physiology · 2024Article
- Review
- From darkness to discovery: evolutionary, adaptive, and translational genetic insights from cavefish.Trends in genetics : TIG · 2024Review
- Trait Loss in Evolution: What Cavefish Have Taught Us about Mechanisms Underlying Eye Regression.Integrative and comparative biology · 2023Review
- Discovery of putative long non-coding RNAs expressed in the eyes of Astyanax mexicanus (Actinopterygii: Characidae).Scientific reports · 2023Article
- Review: Recent Applications of Gene Editing in Fish Species and Aquatic Medicine.Animals : an open access journal from MDPI · 2023Review
- Novel Husbandry Practices Result in Rapid Rates of Growth and Sexual Maturation Without Impacting Adult Behavior in the Blind Mexican Cavefish.Zebrafish · 2023Article
- Natural selection versus neutral mutation in the evolution of subterranean life: A false dichotomy?Frontiers in ecology and evolution · 2023Article
- Characterizing the genetic basis of trait evolution in the Mexican cavefish.Evolution & development · 2022Article
- A chromosome-level genome of Astyanax mexicanus surface fish for comparing population-specific genetic differences contributing to trait evolution.Nature communications · 2021Article
- Dark world rises: The emergence of cavefish as a model for the study of evolution, development, behavior, and disease.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2020Article
- Review
- A Natural Animal Model System of Craniofacial Anomalies: The Blind Mexican Cavefish.Anatomical record (Hoboken, N.J. : 2007) · 2020Article
- Article
- Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry.Journal of visualized experiments : JoVE · 2018Article
- Embryonic origin and genetic basis of cave associated phenotypes in the isopod crustacean Asellus aquaticus.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Astyanax mexicanus, a teleost fish that exists in a river-dwelling surface form and multiple cave-dwelling forms, is an excellent system for studying the genetic basis of evolution. Cavefish populations, which independently evolved from surface fish ancestors multiple times, have evolved a number of morphological and behavioral traits. Quantitative trait loci (QTL) analyses have been performed to identify the genetic basis of many of these traits. These studies, combined with recent sequencing of the genome, provide a unique opportunity to identify candidate genes for these cave-specific traits. However, tools to test the requirement of these genes must be established to evaluate the role of candidate genes in generating cave-specific traits. To address this need, we designed transcription activator-like effector nucleases (TALENs) to target two genes that contain coding changes in cavefish relative to surface fish and map to the same location as QTL for pigmentation, oculocutaneous albinism 2 (oca2) and melanocortin 1 receptor (mc1r). We found that surface fish genes can be mutated using this method. TALEN-induced mutations in oca2 result in mosaic loss of melanin pigmentation visible as albino patches in F0 founder fish, suggesting biallelic gene mutations in F0s and allowing us to evaluate the role of this gene in pigmentation. The pigment cells in the albino patches can produce melanin upon treatment with L-DOPA, behaving similarly to pigment cells in albino cavefish and providing additional evidence that oca2 is the gene within the QTL responsible for albinism in cavefish. This technology has the potential to introduce a powerful tool for studying the role of candidate genes responsible for the evolution of cavefish traits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.